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《Acta Academiae Medicinae Militaris Tertiae》 2006-24
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Effect of implantation of auto-skeletal muscle satellite cells into ischemic myocardium on cardiac function after myocardial infarction in rats

WANG Hong-yong 1,HE Zuo-yun 2,YU Chang-qing 1,XIANG De-bing 1,HU Hou-xiang 1,WANG Yi 1,YANG Cheng-ming 1,WANG Xu-kai 1,FU Chun-jiang 1(1Department of Cardiovascular Diseases,Institute of Surgery Research,Daping Hospital,Chongqing 400042,2Department of Cardiovascular Diseases,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China)  
Objective To investigate the effect of auto-skeletal muscle satellite cell implantation into ischemic myocardium on cardiac function and the mechanisms.Methods Approximately 10 7 to 10 9 muscle satellite cells(SCs)were cultivated in vitro.The left anterior descending(LAD)artery was ligated in Wistar rats to create myocardial infarction(MI).Some rats only underwent sham operation served as control.Two weeks after MI,autologous SCs,serum-free culture medium and sodium chloride injection were injected into ischemic myocardium of implantation rats(n=15),control rats(n=15)and myocardium around LAD of sham operation rats(SO,n=15),respectively.Four weeks after injection,hemodynamic parameters and cardiac function in all groups were evaluated by polygraph system,capillary density in the ischemic myocardium was demonstrated by immunohistochemical method,serum VEGF concentration was examined by ELISA,and the differentiated myofibers from SCs in the infarcted site were observed by pathologic examination and immunohistochemical method.Results Four weeks after injection,the SCs had progressively differentiated into striated muscle fibers in the myocardial infarction site,and immunohistochemical analysis confirmed their skeletal muscle origin.Compared with the SO rats,systolic blood pressure(SBP),diastolic blood pressure(DBP),mean ar-tery pressure(MAP),left ventricular systolic pressure(LVSP)and dp/dtmaxwere markedly decreased(P 0.05 orP0.01)and left ventricular end-diastolic pressure(LVEDP)was markedly increased(P0.01)in the control rats and implantation rats.But the implantation of SCs into ischemic myocardiumof implantation rats can significantly increase +dp/dtmax,-dp/dtmaxand decrease LVEDP as compared with control rats(P0.05 andP0.01).Compared with the SO group,capillary density was markedly increased in the control rats and implantation rats(P0.05,P0.01).Capillary density and serum VEGF concentration in the implantation rats were higher than that in the SO rats and in the control rats(P0.05 orP0.01).Conclusion The SCs,after being implanted into the myocardial infarction site,showed myocardial regeneration and the ability to increase serum VEGF concentration and enhance the capillary density in the ischemic myocardium and finally improve cardiac function.
【CateGory Index】: R542.22
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