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《Acta Academiae Medicinae Militaris Tertiae》 2006-24
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Protective effect of sodium and magnesium saline of fructose-1,6-diphosphate on B cells in pancreatic islet injured by streptozotocin

PENG Dong-hong 1,ZHANG Neng 2(1Department of Respiratory Diseases,Children's Hospital,2Department of Physiology,College of Basic Medicine Chongqing University of Medical Sciences,Chongqing 400014,China)  
Objective To explore whether sodium and magnesium saline of fructose-1,6-diphosphate(Na +-Mg 2+-FDP)protects B cells in islet of Langerhans injured by streptozotocin(STZ).Methods Healthy adult male Wistar rats were randomly divided into five groups:STZ group(receiving once 2% STZ in citric acid solution at dose of 60 mg/kg),blank control group(receiving citric acid solution),FDP group(treated as STZ group,plus Na +-Mg 2+-FDP solution twice a day at total dose of 500 mg·kg-1·d-1 for 15 d),protected group(receiving Na +-Mg 2+-FDP solution at 500 mg/kg first,then 2% STZ in citric acid solution at dose of 60 mg/kg once,Na +-Mg 2+-FDP solution twice a day at total dose of 500 mg·kg-1·d-1 for 15 d),treated group(48 h after receiving 2% STZ in citric acid solution at dose of 60 mg/kg,starting Na +-Mg 2+-FDP solution twice a day at total dose of 500 mg·kg-1·d-1 for 15 d).On the 15 th day after experiment starting,all rats were killed and the end of pancreatic gland was prepared for following experiments.Blood glucose by using glucose oxidase kit and serum insulin by radioimmunoassay was determined.The number of positive islets of which A,B and D cells expressed glucagons,insulin and somatostatin was counted by S-P immunohistochemistry.The morphology of islet of Langerhans stained by HE was observed under optical microscope.Statistical significance was determined by q test and χ 2 test,the related coefficient was determined by rectilinear correlation analysis(α=0.05).Results The blood glucose dropped and serum insulin rose in protected group,but the glucose in treated group and STZ group maintained at high level,and serum insulin maintained at low level.The blood glucose and insulin of the protected group showed significant differences with that of the treated group and STZ group respectively(P0.001).The alteration of blood glucose and insulin showed negative rectiline-ar correlation.In STZ group and treated group,islets shrank,the number of islets decreased,isletcells in large part fused completely and appeared glass-like denaturation,and the cellular nucleus appeared dissolving phe-nomenon.But in protected group,the intact architecture of islet,and very light cellular denaturation was ob-served.The amount of expressing insulin in B cells decreased significantly in STZ group and treated group,and that of expressing glucagons in A cells increased more evidently than that of somatostatin in D cells,and there was no statistical difference between the two groups(P0.05).However,the expressions of insulin,gluca-gons and somatostatin were close to normal level in protected group,which were of statistical significance with that of STZ group and treated group(P0.01 andP0.05).Conclusion Preventive application of FDP has a protective effect on B cells of islets fromthe damaged structure induced by STZ.Therapeutical application of FDP can not protect B cells against destroyed structure.The reason of diabetes occurrence is that B cells are damaged and the insulin reduces.Besides,the increased expression of glucagons and somatostatin may be cor-related closely with diabetes development.
【CateGory Index】: R587.1
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