Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Academiae Medicinae Militaris Tertiae》 2006-24
Add to Favorite Get Latest Update

Detection and significance of MUC1 mRNA in mediastinal lymphnodes and peripheral blood affected by non-small cell lung cancer

ZHENG Shun-li 1,YANG Qing-sheng 1,MA Xiao-hong 2(1Department of Cardiothoracic Surgery,2Surgical Laboratory,The First Affiliated Hospital of Jinan University,Guangzhou 510630,China)  
Objective To discuss the possibility of mucin-1 gene(MUC1 mRNA)in mediastinal lymphnodes(MLNs)and peripheral blood as a symbol of occult metastasis in patients with non-small cell lung cancer(NSCLC).Methods By reverse transcriptase-polymerase chain reaction(RT-PCR),MUC1 mRNA in peripheral blood and in 67 MLNs from 30 patients with benign pulmonary diseases(negative control group)and in 30 MLNs which had metastasis determined by routine pathologic examination(positive control group)was detected;MUC1 mRNA in peripheral blood in 158 patients with NSCLC and in 296 MLNs proven to be tumor-free by routine histopathologic examination from 97 NSCLC patients was detected(experimental group).Results MUC1 mRNA was not identified in peripheral blood in 30 patients with benign pulmonary diseases,but 49 specimens from 158 NSCLC patients were positive(positive rate 31.0%),there was significant difference between these two groups(P0.01).No significant difference between stageⅠand stageⅡ NSCLC(P0.05),but the positive rate of MUC1 mRNA in stage Ⅲ and Ⅳ NSCLC was significantly higher than that in stage Ⅰ and Ⅱ(P0.05 or P0.01).One of 67 benign MLNs was identified to be MUC1 mRNA positive(false positive 1.5%),MUC1 mRNA in 112 MLNs from 296 MLNs in experimental group was positive(37.8%),(P0.05).Conclusion RT-PCR is a specific and sensitive method to detect MUC1 mRNA in MLNs and peripheral blood in NSCLC patients.MUC1 mRNA might be a valuable marker to determine micrometastases in NSCLC patients.It can provide important clinical information for prognosis and selection of appropriate treatment strategies.
【CateGory Index】: R734.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved