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《Acta Academiae Medicinae Militaris Tertiae》 2007-01
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Establishment of scaffold materials of deproteinized heterogeneous bone and their physicochemical properties

LIU Lei,LI Qi-hong,TANG Kang-lai,YANG Liu,JIAN Yue-kui (Department of Joint Surgery,Southwest Hospital,Third Military Medical University,Chongqing 400038,China)  
Objective To establish scaffold materials of deproteinized heterogeneous tissue engineering bone.Methods Deproteinized bones were made through a series of physicochemical treatment from pig ribs and were analyzed with histology,scanning electron microscopy,infrared spectrum,X-ray diffraction analysis,X-ray energy scattering analysis,microdose Kjeldahl determination and mechanical analysis.Results Stain of interstitial collagen fibers was positive and mucin stain was negative in deproteinized bone,but stain of interstitial collagen fibers and mucin were all positive in fresh bone.Deproteinized bone maintained natural network pore system,its pore size was(472.51±7.02) μm,its porosity was(78.15±6.45)%.Infrared spectrum showed collogen in deproteinized bone.Fresh and deproteinized bone were all of curve style atlas of hydroxyapatite,their ratio of calcium to phosphors was(1.71±0.95) and(1.68±0.76)(P0.05),and their protein content was(26.6±2.23)% and(19.1±2.14)%(P0.05).The compression destruction load and destruction maximum load of deproteinized bone were of no significant difference with those of fresh bone((P0.05)),except for high elastic modulus.Conclusion Physicochemical property and mechanical strength of scaffold materials of established deproteinized tissue engineering bone meet request of ideal scaffold materials.Its immunogenicity should be further observed.
【Fund】: 国家高技术研究发展计划重大攻关课题(“863”计划)(2003AA205020)~~
【CateGory Index】: R318.08
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