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《Acta Academiae Medicinae Militaris Tertiae》 2007-01
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Cloning and eukaryotic expression vector construction of mouse FasL gene and its expression in dendritic cells

WANG Yan~(1),ZHUO Wen-lei~(2),WANG Chang-zheng~(1),QIAN Gui-sheng~(1),BI Yu-tian~(1),WU Kui~(1)(~(1)Institute of Respiratory Diseases,~(2)Cancer Research Center,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China)  
Objective To clone mouse FasL(mFasL) full length cDNA and construct eukaryotic expression vector,and to detect its expression in dendritic cells.Methods RT-PCR and TA cloning technique were used to clone the mFasL full length cDNA from mouse activated T lymphocytes,and then eukaryotic expression vector pIRES2EGFP containing mFasL cDNA was constructed.The positive recombinants pIRES2EGFP-mFasL were transfected into dendritic cells(DC),the expression of mFasL mRNA was detected by RT-PCR and the expression of mFasL protein was assayed by immunofluorescence and Western blotting.Results The cloned full reading frame of mFasL cDNA was in coincidence with the sequence registered in GenBank.In the DC after transfection of pIRES2EGFP-mFasL,the expressions of mFasL mRNA and mFasL protein were detected.Conclusion The mFasL cDNA was cloned and constructed into the eukaryotic expression vector successfully,and mFasL gene could be expressed efficiently in DC transfected by pIRES2EGFP-mFasL.
【Fund】: 国家自然科学基金(30470772)~~
【CateGory Index】: Q78
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Chinese Journal Full-text Database 1 Hits
1 XIA Jun-bo, WU Kui, SUN Kun, WANG Chang-zheng (Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medicine University, Chongqing 400037, China);Amplification and identification of dendritic cells from mouse bone marrow[J];Acta Academiae Medicinae Militaris Tertiae;2005-10
Chinese Journal Full-text Database 7 Hits
1 WANG Yan,BI Yu-tian,ZHUO Wen-lei,et al.(1.Institute of Respiratory Diseases of PLA;2.Cancer Research Center,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China);Phenotypes and immune activity of mouse FasL gene-modified dendritic cells[J];Chongqing Medicine;2006-07
2 XIA Jun-bo,SONG Yun-xi,ZENG Xue-feng,WANG Yan,WANG Chang-zheng(Institute of Respiratory Diseases,Xinqiao Hospital,Third Military Medicine University,Chongqing 400037,China);Role of 1,25-Dihydroxyvitamin D_3 treated dendritic cells in allergic airway inflammation[J];Acta Academiae Medicinae Militaris Tertiae;2008-08
3 XIA Jun-bo,SONG Yun-xi,ZENG Xue-feng,WANG Yan,WANG Chang-zheng(Institute of Respiratory Diseases,Xinqiao Hospital,Third Military Medicine University,Chongqing 400037,China);Roles of Th1/Th2 cytokine-treated dendritic cells in allergic airway inflammation[J];Immunological Journal;2008-02
4 FENG Ting,ZHAO Mingyao,SUN Lisha,LI Pei,MA Junfen,HUANG Youtian,DONG Ziming Department of Pathophysiology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001;Effects of achyranthes bidentata polysaccharide on anti-tumor ability of mouse bone marrow-derived dendritic cells[J];Journal of Zhengzhou University(Medical Sciences);2010-03
5 SUN Xin-liu,WANG Jing-bo (Central Laboratory of Taian Central Hospital,Taian 271000,China);Apoptosis effects of murine dendritic cell on CT-26 solid tumor[J];Journal of Taishan Medical College;2008-11
6 WANG Yan*,ZHUO Wen-lei,BI Yu-tian,WU Kui,WANG Chang-zheng,SONG Yun-xi,XIA Jun-bo. *Institute of Respiratory Diseases of PLA,Xinqiao Hospital,Third Military Medical University.Chongqing,400037,China;Effects of FasL gene-modified dendritic cell on the airway inflammation in mice sensitized/challenged by house dust mite allergen[J];Chinese Journal of Respiratory and Critical Care Medicine;2007-01
7 REN Jianlin1 XU Jun2 1.Shanxi Medical University,Taiyuan 030001,China;2.Department of General Surgery,Shanxi Provincial People's Hospital,Taiyuan 030012,China;Effect of TGF-β_1 Receptor Blocker on Fusion Vaccine of Dendritic Cells[J];China Modern Doctor;2010-14
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