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《Acta Academiae Medicinae Militaris Tertiae》 2007-01
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Dynamic changes of apoptotic neuron in hippocampus after pilocarpine induced status epilepticus in rats

ZHANG Ying-qi~(1),LIAO Wei-hong~(2),CHI Lu-xiang~(1),CHEN Kang-ning~1,SHI Shu-gui~1,FAN Wen-hui~1,CHEN Zhen-fang~1 (~(1)Department of Neurology,Southwest Hospital,Chongqing 400038,~(2)State Key Laboratory of Trauma,Burns and Combined Injury,Department 3,Institute of Surgery Research,Daping Hospital,Third Military University,Chongqing 400042,China)  
Objective To probe the occurrence of neuroal apoptosis in rats after status epilepticus(SE) and the relationship between neuron apoptosis and caspase-3 expression.Methods Rat model of SE was induced by intraperitoneal injection of 300 mg/kg pilocarpine in 20 male Wistar rats.Another 5 rats served as control.The apoptotic neuron was detected by TdT-mediated dUTP Nick End Labeling(TUNEL),and caspase-3 expression was observed by immunocytochemistry at 6~(th),48~(th),72~(th) h and 7~(th) d after SE induction.Results In control rats,TUNEL-positive neuron could not be found in hippocampus.The number of TUNEL-positive neuron began to increase at 6~(th) h after SE induction and peaked at 72~(th) h,began to decrease at 7~(th) d after SE induction.In control rats,there were few caspase-3 expression in hippocampus.The caspase-3 immunoreactivity began to increase at 6~(th) h after SE and peaked at 48~(th) h,especially in CA1 and CA3 region of hippocampus,began to decrease at 72~(th) h and dropped to normal level at 7~(th) d.The results indicated that the distribution of caspase-3 expression was consistent with the distribution of TUNEL-positive neuron.The peak of caspase-3 expression was earlier than that of TUNEL-positive neuron.Conclusion Neuron apoptosis participated in the process of delayed neuronal death in hippocampus after SE and was correlated with caspase-3 activation.
【CateGory Index】: R742.1
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