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《Acta Academiae Medicinae Militaris Tertiae》 2007-04
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Effects of IL-2 and IL-10 on T regulatory cell proliferation in vitro

ZENG Dong-zhu 1,WANG Zi-qiang 1,ZHENG Jun-song 2,WU Miao 1,YU Pei-wu 1(1Department of General Surgery,Southwest Hospital,2Department of Clinical Laboratory Science,College of Medical Laboratory,Third Military Medical University,Chongqing 400038,China)  
Objective To explore the promoting effects of IL-2 and IL-10 on Treg proliferation in vitro,and to compare the inhibiting effects of the proliferated Treg on CTL with those of the enriched Treg.Methods After IL-2 or IL-10 was added into the culture system to stimulate Treg proliferation,the Treg cells were counted at 48,96 and 144 h to determine the cytokines' efficacious doses in vitro.After collecting the proliferated Treg,we compared the inhibiting effects of the proliferated Treg on CTL with those of the enriched Treg.Results IL-2 and IL-10 induced Treg proliferation in vitro significantly.IL-2(100 ng/ml)increased the number of Treg cells by 10%,18% and 20% in vitro at 48,96 and 144 h after stimulation.IL-10(150 ng/ml)provided the same inducing effects as IL-2,proving less capable.The proliferated Treg had the same efficacy in inhibiting CTL functions as the enriched Treg(P0.05).Conclusion IL-2 is superior to IL-10 for Treg proliferation in vitro.The proliferated Treg is as efficacious as MACS enriched Treg in inhibiting CTL function.
【Fund】: 国家自然科学基金(30200264 30571863);; 国家自然科学基金重大项目(3993430-2)~~
【CateGory Index】: R392
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 ZHENG Jun song, WU Jun, HUANG Wen hua, PENG Dai zhi, XIAO Guang xia, LUO Zhong hua(Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Effect of severe scalding on function of murine splenic T lymphocytes and their activation by TCR_(α/β) and CD28 signaling pathway[J];Acta Academiae Medicine Militaris Tertiae;2001-12
2 ZHENG Jun-song, WU Jun, YI Shao-xuan, HE Wei-feng, CHEN Xi-wei, XIE Zhi-jie, PENG Shuang-fa( Institute of Bum Research, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Effects of different cytokines and micro-circumstance of thymus on regulatory T cell proliferation in vitro and in vivo[J];Acta Academiae Medicinae Militaris Tertiae;2003-21
3 CUI Ziyou 1), HUANG Youtian 1),ZHAO Jimin 1), ZHAO Jun 1), LU Jing 1), ZHAO Guoqiang 2), ZHAO Mingyao 1), DONG Ziming 1) 1)Department of Pathophysiology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052 2)Department of Microbiology and Immunology,College of Basic Medical Sciences,Zhengzhou University, Zhengzhou 450052;Employing FCM to observe the dynamic changes of T lymphocyte proliferation stimulated by dendritic cells in mice[J];Journal of Zhengzhou University(Science Medical);2005-05
【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 ZHENG Jun song,WU Jun,LUO Gao xing,et al. (Institute of Burns Research,Southwest Hospital,the Third Military Medical University, Chongqing400038);MACS classifacation and the purity identification of regulatory T Cell[J];Chongqing Medical Journal;2002-08
2 LI Min,SONG Li,ZHANG Jian-Bo,FANG Jun,LI Lan.Department of Pediatrics,Sichuan Province People′s Hospital,Sichuan Academy of Medical Sciences,Chengdu 610072,China;Effect of glucocorticoid on CD4~+CD25~+ T regulatory cells in asthmatic mice[J];Chinese Journal of Contemporary Pediatrics;2008-04
3 ZHENG Jun-song, WU Jun, YI Shao-xuan, HE Wei-feng, CHEN Xi-wei, XIE Zhi-jie, PENG Shuang-fa, DAI Fei (Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Immunological mechanism of regulatory T cells acting on effector T cells[J];Acta Academiae Medicinae Militaris Tertiae;2003-21
4 ZHENG Jun-song, WU Jun, YI Shao-xuan, HE Wei-feng, CHEN Xi-wei, XIE Zhi-jie, PENG Shuang-fa( Institute of Bum Research, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Effects of different cytokines and micro-circumstance of thymus on regulatory T cell proliferation in vitro and in vivo[J];Acta Academiae Medicinae Militaris Tertiae;2003-21
5 LI Min,SONG Li,ZHANG Jian-Bo,FANG Jun,LI Lan. Department of Pediatrics,Sichuan Academy of Medical Sciences and Sichuan Province People′s Hospital,Chengdu 610072,China;Changes of CD4~+ CD25~+ regulatory T cells,IL-10 and TGF-β_1 levels in peripheral blood in children with asthma[J];Chinese Journal of Contemporary Pediatrics;2009-10
【Co-references】
Chinese Journal Full-text Database 1 Hits
1 WANG Min, ZHANG Lin-jie, HE Jin-sheng, GE Xiao-song, CHEN Yong, LI Bai-qing(Department of Immunology, Anhui Medical University, Hefei 230001, China);Determination of the proliferation of CFSE labeled human T lymphocyte subpopulations by flow cytometry[J];Immunological Journal;2005-05
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 ZHENG Jun song, WU Jun, HUANG Wen hua, PENG Dai zhi, XIAO Guang xia, LUO Zhong hua(Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Effect of severe scalding on function of murine splenic T lymphocytes and their activation by TCR_(α/β) and CD28 signaling pathway[J];Acta Academiae Medicine Militaris Tertiae;2001-12
2 Yang Hongyan 1) ,Zhang Yiguo 1) ,Dong Ziming 1) ,Zheng Zhimin 1) ,Wu Hao 2) 1)Department of Pathophysiology,Henan Medical University,Zhengzhou 450052 2) Zhengzhou Railway Hospital,Zhengzhou 450052;Isolation and purification of dendritic cells from mouse spleen and bone marrow and their expansion culture induced by cytokines in vitro[J];Journal of Henan Medical University;1999-01
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