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《Acta Academiae Medicinae Militaris Tertiae》 2007-04
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Screening the interaction proteins of MRP2 by yeast two-hybrid system

LIU Tao 1,JIN Chao-zhi 2,ZHANG Cui-li 3,CHEN Hui 4,CHU Zhu-lang 4,WANG Ying-jie 1,YANG Xiao-ming 2(1Institute of Infectious Diseases,Southwest Hospital,Third Military Medical University,Chongqing 40038; 2Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing 100850; 3Department of Procreation Medicine,Chongqing Medical University,Chongqing 400016; 4Department of Pathophysiology,Anhui Medical University,Hefei 230032,China)  
Objective To study interaction proteins of MRP2 by yeast two-hybrid system.Methods Using the 220 C-terminal amino acids of MRP2 as bait,a human liver cDNA library was screened and the proteins interacting with MRP2 were searched.The false clones were eliminated by bioinformatic methods and one to one yeast two-hybrid system.Results Three true positive clones from yeast clones were obtained after verification.Conclusion The encoding proteins we obtained may play important roles in finding the new function of MRP2.
【Fund】: “十五”国家科技攻关计划(2004BA711A19)~~
【CateGory Index】: Q78
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