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《Acta Academiae Medicinae Militaris Tertiae》 2007-04
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Sequence analysis of mtDNA NADH-ubiquinone oxidoreductase chain 4 gene of Asian black bear Sichuan subspecies

CHEN Yu,HOU Wan-ru,WU Xia,PENG Zheng-song,ZHOU Cai-quan(College of Life Science,China West Normal University,Nanchong 637002,China)  
Objective To investigate the sequence characteristic of black bear's mitochondrial NADH-ubiquinone oxidoreductase chain 4 gene(ND4).Methods Primers were designed according to certain species' NADH-ubiquinone oxidoreductase ND4 in which the DNA sequence had been reported.The DNA sequence was amplified by PCR from the Asian black bear Sichuan subspecies's(Ursus thibetanus mupinensis)hair-follicle tissue total DNA and were analyzed with its amino acid sequence.Results The ND4 gene sequence length was 1 402 bp and contained a 1 377-nucleotide open reading frame encoding 459 amino acid residues.The amino acid sequence's pI was 9.37 and weight was 51.5×10 3.The DNA sequence and corresponding amino acid sequence of black bear's ND4 gene had the high similarity with those of other mammalian animals' ND4 gene,and the similarity was 89% to 91% and 94% to 95%.The result of phylogenetic tree was basically consistent with the traditional species system phylogenetic relations.Topology prediction showed the Asian black bear Sichuan subspecies's ND4 proteins contained N-glycosylation site,kinase C phosphorylation sites,casein kinase Ⅱ phosphorylation site,N-myristoylation site and leucine-rich region.In addition,black bear's ND4 protein contained leucine zipper pattern and Leucine-rich region.Conclusion The mammalia animal's ND4 gene has the high conservative nature.The pretein coded by ND4 gene has high uniformity in the function.
【Fund】: 国家自然科学基金(30570275);; 四川省应用技术项目(2006J13-057);; 四川省教育厅重点科研项目(2004A101);; 四川省重点学科建设项目(SZD0420);; 西华师范大学科研启动项目(2003)~~
【CateGory Index】: Q953;Q78
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