Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Academiae Medicinae Militaris Tertiae》 2007-04
Add to Favorite Get Latest Update

Concentration of quinolinic acid in rat serum by GC-MS

XIONG Ying,YU Hua-rong,ZHOU Qi-xin(Department of Pharmacology,College of Pharmacology,Chongqing Medical University,Chongqing 400016,China)  
Objective To establish a sensitive and accurate gas chromatography-mass spectrometry method for determination of quinolinic acid in rat serum.Methods The objective compound was purified and derivatized to its dihexafluoroisopropyl ester before quantitative determination by scanning method and quantitative determination by SIM method.Results The endogenous substances in serum had no interference on the measurement of quinolinic acid in the sample.Assay linearity was obtained in the range of 0.05-20 μmol/L with limit of quantitation(LOQ)of 0.05 μmol/L.The intra-day relative standard deviation(RSD)was lower than 15%.The inter-day relative standard deviation was lower than 12%.Conclusion It is a sensitive,accurate and convenient method.The dihexafluoroisopropyl ester of quinolinic acid is quite stable,but the serum should not be preserved for a long time before derivation.
【CateGory Index】: R446.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 2 Hits
1 ZHOU Qi, WANG Zhengmin EENT Hospital of Shanghai Medical University, Shanghai 200031;Studies on neurotoxic effects of quinolinic acid on spiral ganglion[J];SHANGHAI MEDICAL JOURNAL;1999-05
2 $$$$Guo Qing,Kuang Peigen, Xie Jun Neurotransmitter Laboratory, Kepartment of Neurolty,Chinese PLA General Hospital, Beijing 100853 Quinolinic acid(QUIN),an endogenous metabolite of L- tryptophan,is not only a selective activa- tor of N- methyl-D-asp;Quinolnic acid concentrations in cerebrospinal fluid and plasma of epil eptic patients.[J];;1995-05
【Co-citations】
Chinese Journal Full-text Database 1 Hits
1 XIONG Ying, ZHOU Qi-xin (Chongqing University of Medical Science, Chongqing 400016, China);Development of determination of quinolinic acid in biotic samples[J];Journal of Pediatric Pharmacy;2005-04
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 HUANG Long 1,SONG Xu-yan 1,SUN Hai-hui 2,MA Shu-yi 1 (1 Technology Centre of Wuhan Tobacco Group Co. Ltd.,Wuhan 430051 2 College of Life Sciences,Wuhan University,Wuhan 430072);Analysis and Determination of Chemical Components in Valerian Oil by Gas Chromatography-Mass Spectrometry[J];Amino Acids & Biotic Resources;2002-02
2 Zhan Hongsheng Zhejiang College of TCM,Hangzhou(310009);Experimental Method of Medicinal Serum and Its Application Prospects in the Study and Making of New Chinese Drugs[J];JOURNAL OF ZHEJIANG COLLEGE OF TCM;2000-02
3 LI Ying,XUE Cun kuan,HE Xue bin,et al.Geriatric Institue,Liyuan Hospital,Tongji Medical College,Huazhong Science and Technology University,Wuhan 430077,P.R.China;Using ~(99m)Tc-ECD as the tracer to evaluate the effects of the extract from valeriana officinalis on rat cerebral perfusion[J];Radiologic Practice;2004-02
4 (Ying Jian-bo,Zhang Di./Punlic Security Bureau of Hangzhou,Hangzhou 310004,China);Determination of Ketamine in Urine by Solid Phase Microextraction Coupled with GC Chromatography-Mass Spectrometry(GC/MS)[J];Chinese Journal of Forensic Medicine;2005-06
5 Jia Jianning(Guangxi College of TCM,Nanning 530001 China )BH Zhang Baoheng (Dept. of Pharmacology, Beijing Medical University, Beijing 100083, China);Effects of extract of Valeriana officinalis L. (V_(3d)) on cardiovascular system[J];Journal of Guangxi College of T.c.m;1999-01
6 ZHANG Guo-chang,GAO Ping-jin,SHEN Jie,ZHU Ding-liang(Shanghai Key Laboratory of Vascular Biology,Shanghai Institute of Hypertension,Ruijin Hospital,School of Medicine,Shanghai Jiaotong University,Shanghai 200025,China);Measurement of urine kynurenic acid by high-performance liquid chromatography in SHR[J];Journal of Shanghai Jiaotong University(Medical Science);2007-04
7 Wang Xijun(Heilongjiang University of Traditional Chinese Medicine, Harbin 150040);Studies on Serum Pharmacochemistry of Traditional Chinese Medicine[J];;2002-02
8 ZHANG Yun-bin,TAN Yi-ting (School of Perfumery and Aroma,Shanghai Institute of Technology,Shanghai 200235,China);ISOLATION AND IDENTIFICATION OF POLYPHENOLIC COMPOUNDS FROM POMACE OF IPOMOEA BATATAS L.[J];Food Research and Development;2007-12
9 Yin Hong, Xue Cunkuan, Ye Jianming, at al (Geriatrics Institute, Togji Medical University, Wuhan 430077);An Experimental Study of Valerian Extract Combating Myocardial Ischemia Reperfusion Injury[J];CHINESE JOURNAL OF MICROCIRCULATION;2000-01
10 XUE Cun-kuan, HE Xue-bin, ZHANG Shu-qin, HUANG Xiao-tao(Geriatric Medical and Pharmaceutical Institute of Tongji Medical college of Huazhong Science and Technology University, Wuhan 430077, Hubei, China);Experimental study of anti-tumor effect of valerian iridoids[J];Modern Jorunal of Integrated Traditional Chinese and Western Medicine;2005-15
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Guo Qing, Liu Zuozhou, Liao Weihong (Department of Physiology, the Third Military Medical College, Chongqing);A STUDY OF BURST CHARACTERISTICS OF NORMAL AND EPILEPTOGENIC HIPPOCAMPAL COMPLEX SPIKE CELLS[J];Chinese Journal of Applied Physiology;1990-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved