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《Acta Academiae Medicinae Militaris Tertiae》 2007-04
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Proteomic analysis of lymph nodes affected by esophageal squamous cell carcinoma by two-dimensional gel electrophoresis

WANG Bin 1,LI Jun-cai 2,FU Zhong-xue 2,ZHU Dan 3(1Department of Cardiothoracic Surgery, 2Department of General Surgery,The First Affiliated Hospital, 3Laboratory of Clinical Diagnostics,Chongqing Medical University,Chongqing 400016,China)  
Objective To analyze the differential expression of proteome between metastatic lymph nodes and normal lymph nodes in esophageal squamous cell carcinoma.Methods Two dimensional polyacrylamide gel electrophoresis(2-DE)technique and computer-assisted image analysis were used to separate the protein spots and analyze the differential expression of proteome in the metastatic lymph nodes and normal lymph nodes.Results There were 23 protein spots significantly differentially expressed,of which 4 protein spots were newly found,1 protein spot disappeared,14 protein spots increased markedly,and 4 protein spots decreased significantly.Conclusion The proteomic expression in metastatic lymph nodes of esophageal squamous cell carcinoma is significantly different from that of the normal lymph nodes,which may be associated with the mechanism of metastasis of esophageal squamous cell carcinoma.
【Fund】: 重庆市自然科学基金(2006BB5302)~~
【CateGory Index】: R735.1
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