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《Acta Academiae Medicinae Militaris Tertiae》 2007-12
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Function of TIMP-3 in arteriosclerosis of orthotopic transplant during chronic rejection

LI Yue1,GONG Jian-ping1,LIU Chang-an1,ZHAO Lei1,GAN Lin2(1Department of Hepatobiliary Surgery,The Second Affiliated Hospital,Chongqing Medical University,Chongqing 400010,2Chongqing Tumor Institute,Chongqing 400030,China)  
Objective To investigate the function of TIMP-3 in the development of graft arteriosclerosis after orthotopic transplantation.Methods SD-Wistar abdominal aorta transplantation was performed in 60 pairs,30 pairs of which received the exogenous TIMP-3 transfection into transplanted vessels.Wistar-Wistar abdominal aorta transplantation was performed in 30 pairs.The expressions of MMP-1,2 and TIMP-3 in the tissues of transplanted abdominal aorta were measured by immunohistochemical method,the expression of MMP-2 was detected by Northern blot quantitative analysis and the activity of MMP-2 was observed by photo analysis on 3rd day,1st,2nd,4th,8th week after transplantation.Results The expressions of MMPs,TIMP-3 in the allograft group were higher than the isograft group.The expressions of TIMP-3 on 1st and 2nd week [(1.7±1.1) and(0.9±0.4)] in the allografts with exogenous TIMP-3 gene transfection were much higher than those of allografts and isografts(P0.05),but the activity of MMP-2 was opposite.Conclusion While the exogenous TIMP-3 gene was admitted on early stage of CR,intervening the balance between MMPs and TIMPs,decreasing the expression and activity of MMPs,and reducing the formation of ECM can slacken CR efficiently.
【Fund】: 重庆市卫生局医学科研基金(渝卫医科07-2-187)~~
【CateGory Index】: R617
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 ZENG Zhong, HAN Ben li (Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Effects of antisense TIMP1 gene on extracellular matrix metabolism in Ito cells in vitro[J];Acta Academiae Medicine Militaris Tertiae;2002-06
2 GE Cheng qun, LI Qian sheng, JIN Feng shuo,JIANG Jun, GE Cheng guo, ZHANG Li bo (Department of Surgery, No. 3 People's Hospital of Linyi City, Linyi 276034, China);Expressions and significance of PDGF and IGF-1 in rat aortic allografts[J];Acta Academiae Medicine Militaris Tertiae;2002-07
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Wu Yunjin, Zhai Weirong, Zhuang Li, Zhang Yue'e, Zhu Hongguang (Department of Pathology,School of Basic Medical Science,Shanghai Medical University,Shanghai 200032);Expression of Matrix Metalloproteinase2 and It's Tissue Inhibitor in Rat Liver Fibrosis Model[J];JOURNAL OF SHANGHAI MEDICA(UNIVERSITY);1999-04
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