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《Acta Academiae Medicinae Militaris Tertiae》 2008-01
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LPS up-regulates corticotropin releasing hormone and urocortin in cultured rat peritoneal macrophages

YANG Lie, ZHOU Ji-hong, ZHANG Xiu-zhu, LIU Da-wei, SUN Qiao-ling, JIANG Jian-xin (State Key Laboratory of Trauma, Burns and Combined Injury, Department 4, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China)  
Objective To investigate the synthesis of corticotropin releasing hormone (CRH) and urocortin (Ucn), in rat peritoneal macrophages and the effect of LPS on their expressions. Methods Peritoneal macrophages were harvested from Wistar rats, and cultured in DMEM with 10% fetal bovine serum. The total RNA and total protein were extracted from normal control cells and the cells treated by LPS for 1, 2, 4 and 8 h. Each cell group was composed of 8 samples from different rats RT-PCR was used to observe transcriptions of CRH mRNA and Ucn mRNA. ELISA was used to observe the expression of intracelluar CRH and Ucn. Results CRH and Ucn mRNA existed in normal rat peritoneal macrophages and LPS up-regulated them to the peak at 4 h. CRH and Ucn peptides also existed in normal rat peritoneal macrophage and they respectively reached the peaks (0.30±0.07) ng/107 cells at 2 h and (8.68±1.25) ng/107cells at 8 h after LPS treatment. Conclusion LPS enhancet the expressions of CRH and Ucn in cultured rat peritoneal macrophages, but Ucn peptide level is higher than CRH peptide. These results indicate the macrophages are the potential resource of CRH and Ucn in inflammatory reaction.
【Fund】: 国家重点基础研究发展规划项目(“973”项目)(2005CB522602)~~
【CateGory Index】: R346
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【References】
Chinese Journal Full-text Database 1 Hits
1 WANG Wu-chao,CHEN Jin-ping,ZHANG Xiu-zhu,DAI Wei,LIU Da-wei,ZHOU Ji-hong(State Key Laboratory of Trauma,Burns and Combined Injury,Department 4,Institute of Surgery Research,Daping Hospital,Third Military Medical University, Chongqing 400042,China);Effects of corticotrophin-releasing hormone on IL-10 secretion from peritoneal macrophages and its signal mechanism[J];Acta Academiae Medicinae Militaris Tertiae;2009-22
【Citations】
Chinese Journal Full-text Database 1 Hits
1 ZHANG De-ming~ 1 , DENG Qing-nan~ 2 , LI Yong-wang~ 1 , MAO Bao-ling~ 1 , QIAN Gui-sheng~ 1 ( ~ 1 Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, ~ 2 Department of Gerontological Respiratory Guangzhou General Hospital of Guangzhou Military Command Area, Guangzhou 510010, China);Effects of polyclonal lipopolysaccharide binding protein antibody on the expression of Toll-like receptor 4 and tolerance to lipopolysaccharide in alveolar macrophages of rats[J];Acta Academiae Medicinae Militaris Tertiae;2006-02
【Co-citations】
Chinese Journal Full-text Database 1 Hits
1 FENG Qi-jia1,XU Zhi1,WU Guo-ming1,HU Chuan-min2,XU Shun-gui1,CHEN Wei-zhong1,GUO Rui-ling1 (1Institute of Respiratory Diseases,Xinqiao Hospital,Chongqing 400037,2Department of Clinical Biochemistry,College of Medical Laboratory,Third Military Medical University,Chongqing 400038,China);Prediction of B-cell antigen epitope on lipopolysaccharide-binding protein[J];Acta Academiae Medicinae Militaris Tertiae;2008-04
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 ;Tolerance of lipopolysaccharide related to the expression changes of TLR4 mRNA[J];Acta Academiae Medicinae Militaris Tertiae;2003-05
2 ZHANG De ming 1, LI Yong wang 2, MAO Bao ling 2, QIAN Gui sheng 2 ( 1Department of Respiratory Diseases, General Hospital of Guangzhou Military Area Command, Guangzhou 510010, 2Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China);Effects of polyclonal antibody to lipopolysaccharide binding protein on IL-10 and IL-18 mRNA expressions of alveolar macrophages in lipopolysaccharide-induced acute lung injury in rats[J];Acta Academiae Medicinae Militaris Tertiae;2004-10
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