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《Acta Academiae Medicinae Militaris Tertiae》 2008-01
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Subclinical seizure and mossy fiber sprouting in hippocampus of rats following hypoxic brain damage

CHEN Xiu1, WU Wan-fu1, HU Chang-1in1, CAI Wen-qin2, YANG Zhong2 (1Department of Neurology, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010; 2Department of Neurobiology, College of Medicine, Third Military Medical University, Chongqing 400038, China)  
Objective To explore the pathophysiological mechanism of subclinical seizures following hypoxic brain injury. Methods Totally 71 adult male SD rats were set in a close chamber with 8% nitrogen oxygen ventilation (0.5 L/min) till they lost breath every day for 7 d to induce hypoxic brain injury. Another 9 rats living in normal air condition served as control. Electroencephalography was carried out 20 min before the hypoxia and 2 h after hypoxic ventilation to detect subclinical seizures. The animals were sacrificed in 7, 14, and 28 d after the end of hypoxia. Mossy fiber sprouting in the hippocampus were detected by Timm,s histochemical staining. Results Subclinical seizures occurred in 19.67% rats (14/71) following hypoxia. In Sub-clinical seizure group, brownish black granule sediment appeared in the pyramidal layer of CA3 region in 7 d following hypoxia, and became more intensive in bundle-like or patch-like way in 14 d, moreover, an obvious stained band was formed in 28 d. Compared with non-subclinical seizure group and control group, the scores of mossy fiber sprouting in the hippocampal CA3 region were higher in subclinical seizure group. But there was no significant difference of mossy fiber sprouting in dentate gyrus among the 3 groups above. Conclusion Subclinical seizures may occur following hypoxia in adult rats, and prominent mossy fiber spouting is formed in the hippocampal CA3 region of these rats, which may be related to seizure following hypoxic brain damage .
【CateGory Index】: R742.1
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