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《Acta Academiae Medicinae Militaris Tertiae》 2008-05
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Expressions of P56,IFN-α and IFN-β mRNAs in major organs of severe burn mice and effects of KPV and anaesthesia

LI Shu-rong1, SU Yong-ping2, DENG Zheng-yang2, LOU Shu-fen2, CHENG Tian-min2 (1Department of Pathology, Chengdu Medical College, Chengdu 610083; 2State of Key Laborary of Trauma, Burn and Combined Injury, Department of Radiation Medicine, Institute of Combined Injury of PLA, Third Military Medical University, Chongqing 400038, China)  
Objective To investigate the expressions of P56, IFN-β, IFN-α mRNAs and the effects of KPV, a COOH-terminal tripeptide sequence of alpha-melanocyte stimulating hormone [alpha-MSH(11-13)], and anaesthesia on the expressions in severe burn injured mice.Methods Eighty mice were randomly and equally divided into 4 groups: control, burn (TBS15%Ⅱ), burn+KPV (injection of 40 μg per animal immediately after burn), and burn+anaesthesia (injection of 0.4 mg/kg diazepam and 10 mg/kg ketamine immediately after burn). All animals were sacrificed in 3 h after burn respectively. The expressions of P56, IFN-β, and IFN-α mRNAs in liver, spleen, lung and kidney tissues were detected with RT-PCR. Results P56 was expressed highly in liver, spleen, lung, and kidney in normal control group, and was obviously increased in kidney in burn group and burn+KPV group, but that in burn+anaesthesia group had no significant difference with that in normal control. IFN-β was not expressed in live and lightly expressed in spleen, lung and kidney, and its expression in lung was significantly increased in burn group and burn+anaesthesia group, but mildly decreased in burn+KPV group. IFN-α mRNA was expressed in all detected organs in normal control, and highly expressed in kidney of burn group and burn+KPV group, but not in burn+anaesthesia group. Conclusion These results indicate that the increased IFN expressing induce the up-regulation of P56, suggesting which may be involved in the disorder of early post trauma stress.
【Fund】: 国家重点基础研究发展规划项目(“973”项目)(G1999054201)~~
【CateGory Index】: R644
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【Co-citations】
Chinese Journal Full-text Database 7 Hits
1 ZHONG He-jiang 1,YANG Tian-de 1,SU Yong-ping 2(1.Department of Anesthesiology,Xinqiao Hospital,the Third Military University,Chongqing 400037,China;2.State key laboratory of trauma,Burn and combined Injury Institute of Combined Injury of PLA,the Third Military Medical University,Chongqing 4000038,China);Effects of JAB1 and LPS on glucocorticoid receptor transcription activation[J];Chongqing Medical Journal;2004-11
2 WANG Jun ping, SU Yong ping, ZHAO Jing hong, QIN Rong (Institute of Combined Injury, Third Military Medical University, Chongqing 400038, China);Reasonable usage of ectogenous glucocorticoids for the management of severely scalded rats[J];Acta Academiae Medicinae Militaris Tertiae;2003-05
3 WANG Ming-hai, SU Yong-ping, CHENG Tian-min, WANG Feng-chao, LU Jian-hua, LIU Xiao-hong, LI Hong-tao (State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China);Inhibition of glucocorticoid receptor α using hairpin siRNA expression vector in vitro[J];Acta Academiae Medicinae Militaris Tertiae;2004-20
4 GUO Xiao-shu1,2,LI Hong-tao1,FANG Hai-li2,WANG Jun-ping1,SU Yong-ping1(1State Key Laboratory of Trauma,Burns and Combined Injury,Institute of Combined Injury,College of Preventive Medicine,Third Military Medical University,Chongqing 400038;2Department of Physiology,School of Preclinical Medicine,Chongqing Medicine University,Chongqing 400016,China);Preparation of polyclonal antibody of mouse IFIT1[J];Acta Academiae Medicinae Militaris Tertiae;2007-15
5 GUO Xiao-shu1,2, FANG Hai-li2, LI Jun1, SU Yong-ping1 (1State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University; Chongqing 400038; 2Department of Physiology, School of Preclinical Medicine, Chongqing Medical University,Chongqing 400016,China);Effect of cervical sympathetic block on hepatic interferon-induced protein IFIT1 in burned mice during early stage[J];Acta Academiae Medicinae Militaris Tertiae;2007-17
6 DING Xin, HOU Ying, JU Yan, SHI Heng-Jun Department of Traditional Chinese Medicine, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China;Effect of Nourishing Yin Antitoxic Capsule 2 on glucocorticoid receptor protein expression in Yin deficiency rats induced by hydrocortisone[J];Journal of the Fourth Military Medical University;2006-23
7 GONG Ji-Ping1,2,CHEN Yu-Pei2,GUO Xiao-Shu3,SU Yong-Ping11PLA Institute of Combined Injury,& State Key Laboratory of Trauma,Burns and Combined Injury,Third Military Medical University,Chongqing 400038 China,2Department of Anesthesia,School of Second Clinical Medicine,Chongqing Medical University,Chongqing 400010,China,3Department of Physiology,School of Preclinical Medicine,Chongqing Medical University,Chongqing 400016,China;Effect of cervical sympathetic block on Interferon-induced protein IFIT1 levels in organs of burned mice during early stage[J];Journal of the Fourth Military Medical University;2009-06
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 LIU Du hu, SU Yong ping, LOU Shu fen, ZHANG Wei, GAO Jing sheng, CHENG Tian min (Department of Radiation Medicine, Institute of Combined Injury of PLA, Third Military Medical University, Chongqing 400038, China);Changes of glucocorticoid receptor in hepatic cytoplasm in scalded rats and its regulation by α-MSH and KPV peptide[J];Acta Academiae Medicine Militaris Tertiae;2001-05
2 WANG Zhi\|hong 1, XIAO Su\|fang 2, LIN Hai 2 (1.Department of Physiology, Fujian Medical University, Fuzhou 350004,China 2.Medical School of Fuzhou Millitary Region,PLA, Fuzhou 350003, China);Diminution of Glucocorticoids Receptors and Inflammatory Response Syndrome in Rats after Severe Scald[J];JOURNAL OF FUJIAN MEDICAL UNIVERSITY;2000-01
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