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《Acta Academiae Medicinae Militaris Tertiae》 2008-05
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Cytocompatibility of biomaterial poly (DL-lactide-co-glycolide) with olfactory ensheathing cells

JIAO Shu-sheng1, LI Bing-cang1, CHEN Jian-mei2, YOU Hua1 (1State Key Laboratory of Trauma, Burn and Combined Injury, Department of 6, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042; 2Fuzhou General Hospital of Nanjing Command, Fuzhou 350001, China)  
Objective To investigate the cytocompatibility between poly (DL-lactide-co-glycolide) (PLGA) and olfactory ensheathing cells (OECs) so as to found a basis to prepare an artificial nerve made of PLGA with OECs. Methods Freshly isolated OECs were seeded on PLGA membrane, and then their growth and adhesion to the membrane were observed at different time points by phase contrast inverted microscopy and scanning electron microscopy. The proliferation and the cell cycle of OECs were examined by MTT and flow cytometry. Results There was no statistically difference between the morphological feature, proliferative capacity and cell cycle of OECs cultured on PLGA membrane and those normally cultured (P0.05). Conclusion The PLGA processes good compatibility with OECs, and it is suitable to construct tissue engineering artificial nerve with OECs.
【Fund】: 国家重点基础研究发展规划项目(“973”项目)(2005CB522604)~~
【CateGory Index】: R318.08
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