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《Acta Academiae Medicinae Militaris Tertiae》 2008-05
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Re-endothelialization of VEGF-transfected endothelial progenitor cells in rat artery intimal injury model

ZHAO Gang, HUANG Lan, SONG Yao-Ming, ZHU Guang-Xu, SONG Ming-Bao (Department of Cardiology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China)  
Objective To explore the proliferation of endothelial progenitor cells (EPCs) after the transfection of human VEGF165, and investigate the re-endothelialization of the transfected cells in artery intimal injury rats. Methods After isolated from rat bone marrow, EPCs were cultured and then identified by FITC-UEA-I and Dil-acLDL. EPCs cells were transfected with pcDNA3-hVEGF165 or pcDNA3 by DOTAP Liposome. After transfection, the expression of VEGF was detected by using ELISA.The EPCs proliferation was studied by MTT methods. VEGF-transfected EPCs were transplanted into experimental artery intimal injury rats and its effect on injured vascular re-endothelial was studied. Results Cells expressed double FITC-UEA-I and Dil-acLDL fluorescence were confirmed to EPCs. The medium of EPCs transfected with pcDNA3-hVEGF165 had expression of VEGF. VEGF165 gene transfer stimulated EPCs adhesion, migration and proliferation. Gene-modified EPCs transplanted into rats, survived in intimal vascular injury site, repaired intimal vascular injury better than those in normal EPCs group. Conclusion VEGF165 gene is successfully transfected into EPCs, which can stimulate EPCs’ migration, adherence and proliferation. VEGF-transfected EPCs have stronger intimal repair ability compared with non-transfected EPCs. VEGF-transfected EPCs repair injured vascular intima, restore normal vascular endothelial structure.
【Fund】: 国家自然科学基金(30470729)~~
【CateGory Index】: R363
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1 YANG Long1,YANG Xin-chun2,YANG Jun-ke3. 1.Department of cardiology,Zunyi Hospital,Zunyi Guizhou 563002;2.Center of Cardiology,Beijing Chaoyang Hospital of Capital Medical University,Beijing 100000;3.Department of Cardiology,the General Hospital of Second Artillery of PLA,Beijing 100000;China;Vascular Endothelial Growth Factor Promotes the Proliferative Capacity of Endothelial Progenitor Cells by Increasing Calcineurin Enzymatic Activity[J];Chinese Journal of Hypertension;2009-03
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