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《Acta Academiae Medicinae Militaris Tertiae》 2008-10
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Verification of differential expression of GBLP in HUVEC treated by R/S-propranolol

MAO Xiao-qin1,QIU Feng2,QIU Zong-yin1,CHEN Shi-zhi3,JIA Xiong-fei4(1Department of Laboratory Medicine,Key Laboratory of Laboratory Medical Diagnostics of Ministry of Education,2The First Affiliated Hospital,Chongqing 400016,3The Second Affiliated Hospital,Chongqing Medical University,Chongqing 400010;4State Key Laboratory of Trauma,Burns and Combined Injury,Institute of Burns,Southwest Hospital,Third Military Medical University,Chongqing 400038,China)  
Objective To verify the differential expression of the protein-guanine nucleotide-binding protein subunit beta 2-like 1(GBLP)that has been identified by comparative proteomics methods.Methods Human umbilical vein endothelial cells(HUVEC)were treated with R/S-propranolol(R/S-PRO)respectively for 3 h,then the differentially expressed GBLP protein was isolated and identified by comparative proteomics methods.Western blot analysis and the confocal laser scanning microscopy(LSCM)were used to further verify the expression abundance and display location of GBLP in two enantiomers treated HUVEC.Results Both results of Western blot and LSCM exhibited that the expression abundance of GBLP in S-PRO treated HUVEC was lower than that in R-PRO treated cells.GBLP located in cytoplasm and plasma membrane in HUVEC,homogeneously distributed in cytoplasm and aggregatively distributed in plasma membrane.Conclusion The differential expression of GBLP proven by Western blot and LSCM is consistent with that identified by proteomics methods.
【Fund】: 重庆市自然科学基金(2005BB5227)~~
【CateGory Index】: R329
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 LIU Hong-Yan, CONG Yu-Wen~*, CHEN Bo, SHAN Ya-Jun, YANG Zhen, ZHAO Zhen-Hu, MAO Bing-Zhi(Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850,China);Screening and identification of a new Mpl-interacting protein, RACK1[J];Bulletin of The Academy of Military(Medical Sciences);2005-03
【Co-citations】
Chinese Journal Full-text Database 1 Hits
1 QIU Feng~ 1) , MAO Xiao-Qin~ 2) , CHEN Shi-Zhi~ 3) , QIU Zong-Yin~ 4) , JIA Xiong-Fei~ 5) ( ~ 1) First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China; ~ 2) First Peoples Hospital of Yunnan Province,Kunming 650032,China; ~ 3) Second Affiliated Hospital,Chongqing Medical University,Chongqing 400010,China; ~ 4) Key Laboratory of Laboratory Medical Diagnostics of Ministry of Education,Chongqing Medical University,Chongqing 400016,China; ~ 5) Kunming, PLA General Hospital,Kunming 650032,China);Screening of GBLP-interacting Proteins with Co-immunoprecipitation and an Integrated Microfluidic System[J];Chinese Journal of Biochemistry and Molecular Biology;2009-03
China Proceedings of conference Full-text Database 1 Hits
1 QIU Feng,MAO Xiao-Qin 2),CHEN Shi-Zhi,QIU Zhong-Yin 4)*,JIA Xiong-Fei5)1)3)(1)the First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China;2)Key Laboratory of Laboratory Medical Diagnostics of Ministry of Education,Chongqing Medical University,Chongqing 400016,China;3)the Second Affiliated Hospital,Chongqing Medical University,Chongqing 400010,China);Screening and identification of GBLP interactional proteins[A];[C];2008
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