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《Acta Academiae Medicinae Militaris Tertiae》 2008-10
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Effects of ceramide on apoptosis of human endothelial cells and expressions of bcl-2 and bax

SHEN Zhu-fu,YANG Li-xia,GUO Rui-wei,YANG Dong,QI Feng,SHI Yan-kun(Department of Cardiology,Kunming General Hospital,Chengdu Military Command,Kunming 650032,China)  
Objective To observe the effect of ceramide on apoptosis of endothelial cells(EC)and determine the relationship between the ceramide-induced growth suppression and the expressions of bax and bcl-2.Methods Human umbilical endothelial cells were cultured in vitro and treated with 5,12.5,25,50 μmol/L C2-ceramide,the cells treated with absolute alcohol as control.TUNEL was employed to determine the apoptosis of the cultured EC.Bax and bcl-2 mRNA and protein levels of EC were detected by RT-PCR and Western blot.Results The number of apoptotic cells was significantly higher in ceramide-treated endothelial cells than that in the control group(P0.05).Ceramide-induced EC apopotsis was in time-and dose-dependent manner.Compared with the control cells,the expressions of bax mRNA and protein in EC treated with ceramide significantly increased(P0.05),while the expressions of bcl-2 mRNA and protein significantly depressed(P0.05).Conclusion Ceramide may induce apoptosis of endothelial cells by down-regulating bcl-2 and up-regulating bax.
【CateGory Index】: R329
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