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《Acta Academiae Medicinae Militaris Tertiae》 2008-10
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Effects of ceramide on apoptosis of human endothelial cells and expressions of bcl-2 and bax

SHEN Zhu-fu,YANG Li-xia,GUO Rui-wei,YANG Dong,QI Feng,SHI Yan-kun(Department of Cardiology,Kunming General Hospital,Chengdu Military Command,Kunming 650032,China)  
Objective To observe the effect of ceramide on apoptosis of endothelial cells(EC)and determine the relationship between the ceramide-induced growth suppression and the expressions of bax and bcl-2.Methods Human umbilical endothelial cells were cultured in vitro and treated with 5,12.5,25,50 μmol/L C2-ceramide,the cells treated with absolute alcohol as control.TUNEL was employed to determine the apoptosis of the cultured EC.Bax and bcl-2 mRNA and protein levels of EC were detected by RT-PCR and Western blot.Results The number of apoptotic cells was significantly higher in ceramide-treated endothelial cells than that in the control group(P0.05).Ceramide-induced EC apopotsis was in time-and dose-dependent manner.Compared with the control cells,the expressions of bax mRNA and protein in EC treated with ceramide significantly increased(P0.05),while the expressions of bcl-2 mRNA and protein significantly depressed(P0.05).Conclusion Ceramide may induce apoptosis of endothelial cells by down-regulating bcl-2 and up-regulating bax.
【CateGory Index】: R329
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【References】
Chinese Journal Full-text Database 1 Hits
1 GAO Li,ZHANG Xi,GAO Lei,CHEN Xing-hua(Department of Hematology,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China);Effect of stromal cells derived from different origins on proliferation and apoptosis of KM3 cells[J];Acta Academiae Medicinae Militaris Tertiae;2009-10
【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 ZHANG Ping1,YUAN Li-jie1,ZHAO Lei1,LI Bai-xiang2(1.Department of Biological Chemistry,Daqing Campus of Harbin Medical University,Daqing 163319,Heilongjiang;2.Department of Toxicology,Public Health College,Harbin Medical University,Harbin 150081,Heilongjiang,China);Effect of Sphingomyelin Hydrolysates on Alkaline Sphingomyelinase Expression in Human Cancer HT-29 Cell[J];Carcinogenesis,Teratogenesis & Mutagenesis;2009-06
2 GAO Yong\|lin, ZHENG Da\|li, HE Yan, LIN De\|xin Division of Biochemistry and Molecular Biology, Fujian Medical University, Fuzhou 350004, China;Apoptosis of Hepatoma Cells Induced by C_2-Ceramide[J];Journal of Fujian Medical University;2003-02
3 YU Zhan cai, LI Zhen guang, WANG Yuan chen (Wendeng Central Hospital, Weihai 264400, China);Critical role of phospholipids in apoptosis[J];Basic Medical Sciences and Clinics;2003-01
4 Shen Zhufu,Yu Dewen,Chen Dailu,Yang Yu,Tan Zhisheng,Wang Xiangping,Liang Yuan,Liu Dan Department of Cadres,Kunming General Hospital of Chengdu Military Command,Kunming,Yunnan,650032,China;Ceramide induces apoptosis of human endothelium via up-regulation of bax expression[J];Medical Journal of National Defending Forces in Southwest China;2010-02
5 LI Xiao-ping,WANG Jian-liu;Medication protect against chemotherapy-induced ovarian injury[J];Chinese Journal of Practical Gynecology and Obstetrics;2010-03
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