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《Journal of Third Military Medical University》 2012-01
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Silencing pregnane X receptor improves chemosensitivity of colon cancer LS174T cells to oxaliplatin

Jiang Heng1,2,Chen Jian2,Chen Keli1,Li Jianjun1,Pan Feng1,Liang Houjie1(1 Department of Oncology,Southwest Hospital,Third Military Medical University,400038,Chongqing;2Department of Oncology,No.452 Hospital of PLA,Chengdu,Sichuan Province,610021,China)  
Objective To determine the effect of silencing pregnane X receptor(PXR) expression by RNA interference on the chemosensitivity of LS174T cells to oxaliplatin.Methods The PXR RNAi expression plasmids were constructed,and then transfected LS174T cells to establish stable transfection cell clones.The expression of specificity protein(SP) 1 and multidrug resistance-associate protein 3(MRP3) were detected by RT-PCR and Western blotting.The chemosensitivity of the transfected and nontransfected cells to oxaliplatin was evaluated by MTT assay.Results The cell clones PXRi 1# and PXRi 2# with stable PXR silence were constructed successfully.In cell clones PXRi 1# and PXRi 2#,the expression of MRP3 at mRNA and protein levels was decreased markedly,and that of SP1 also was decreased.Compared with control group,the IC50 of PXRi 1# and PXRi 2# cells to oxaliplatin was significantly decreased(4.83±0.22 and 4.75±0.19 vs 9.44±0.37,P0.05).Conclusion Interference of PXR expression can elevate the chemosensitivity of LS174T cells to oxaliplatin,which might be through PXR knock-down downregulating SP1 and MRP3.
【Fund】: 国家自然科学基金面上项目(81172114);; 四川省卫生厅科研课题(100188)~~
【CateGory Index】: R735.35
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