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《Journal of Third Military Medical University》 2012-01
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Effect of RNAi-mediated gene silencing of HER2/neu on proliferation and apoptosis in bladder cancer cell line BIU-87

Li Yongsheng1,Zhao Cheng1,Liu Jinyu2,Hu Zhao1,Huang Yuqing1(1Department of Urological Surgery,Union Clinical College of Fujian Medical University,Fuzhou,Fujian Province,350001;Department of Urological Surgery,Affiliated Hospital of Putian College3,Putian,Fujian Province,351100,China)  
Objective To investigate the effect of small interfering RNA(siRNA)-mediated gene silencing of HER2/neu on the proliferation and apoptosis in bladder cancer cell line BIU-87.Methods HER2/neu siRNA was chemically synthesized and transfected into BIU-87 cells mediated by liposome.BIU-87 cells with different treatments were divided into an HER2/neu siRNA group,an empty liposome group,a negative siRNA group and a non-transfection group was the control group.Inhibitory effect of HER2/neu siRNA on BIU-87 cell proliferation was assessed by CCK-8 assay.Cell apoptosis was examined by flow cytometry(FCM).mRNA and protein levels of HER2/neu in BIU-87 cells before and after transfection were detected by reverse transcription-polymerase chain reaction(RT-PCR) and Western blotting.Results BIU-87 cells transfected with HER2/neu siRNA showed a significant decrease of cell viability from(82.37±0.90)% to(56.76±1.70)%(P0.05) and cell apoptosis with a rate of(45.60±0.70)%,which was significantly different from that of the control group,empty liposome group and negative HER2/neu siRNA group(P0.05).The siRNA targeting HER2/neu gene significantly reduced mRNA and the protein expressions of HER2/neu in BIU-87 cells(P0.05).Conclusion Chemically synthesized siRNA targeting HER2/neu gene,which can effectively inhibit HER2/neu gene expression and subsequently inhibit cell proliferation and induce apoptosis in BIU-87 cells,is a potential target for gene therapy of bladder cancer.
【Fund】: 福建省科技厅青年人才项目(2007F3036)~~
【CateGory Index】: R737.14
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