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《Journal of Third Military Medical University》 2012-01
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Soy isoflavones promotes apoptosis in human prostate cancer DU-145 cells through PPARγ activation

Wei Hongmei,Zhou Jing,Rong Rong,Zhu Jundong(Department of Nutrition and Food Hygiene,Chongqing Medical Nutrition Research Center,Chongqing Key Laboratory of Nutrition and Food Safety,College of Military Preventive Medicine,Third Military Medical University,Chongqing,400038,China)  
Objective To investigate the effect of genistein(GEN) and daidzein(DAI) on apoptosis in human prostate cancer DU-145 cells and their correlation with peroxisome proliferators-activated receptor γ(PPARγ).Methods PPARγ activity in prostate cancer DU-145 cells treated with soy isoflavones was mea-sured by peroxisome proliferator responsive element(PPRE)-driven luciferase reporter gene assay.DU-145 cells were respectively treated genistein and daidzein alone and either of them combined with GW9662,which was a selective inhibitor of PPARγ,for 48 h.The expression and distribution of PPARγ were detected by immunofluorescence staining.Cell apoptosis was determined by TUNEL assay and AnnexinⅤ/PI dual-color flow cytometry.Results Genistein and daidzein significantly enhanced luciferase expression activity in DU-145 cells transfected with PPRE-TK-Luc plasmid,and the effect could be reversed by GW9662.The changes of PPARγ expression in DU-145 cells were not significant after treatment with either genistein or daidzein,but PPARγ translocation to nucleus increased.The apoptotic rate of DU-145 cells treated with genistein or daidzein increased significantly(P0.05).When cells were treated with either genistein or daidzein combined with GW9662,the increased apoptosis rate and PPARγ nucleus translocation were significantly inhibited.Conclusion Soy isoflavones promote apoptosis in human prostate cancer DU-145 cells through PPARγ activation.
【Fund】: 国家自然科学基金(30771793)~~
【CateGory Index】: R285
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