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《Journal of Third Military Medical University》 2012-01
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Effect of crosstalk between EGFR and integrin signaling pathways on invasion of gastric cancer cells

Ding Jian1,Li Dan2,Lin Na2,Wang Xiaozhong2,Wang Chengdang1,Wu Ting1(1Department of Enterology,First Affiliated Hospital,Fujian Medical University,Fuzhou,Fujian Province,350005;2Department of Enterology,Union Hospital of Fujian Medical University,Fuzhou,Fujian Province,350001,China)  
Objective To understand crosstalk between epidermal growth factor receptor(EGFR) and integrin signaling pathways and effect of the crosstalk on cell invasion and proliferation in human gastric cancer cell SGC7901.Methods Human gastric cancer cells SGC7901 were stimulated with EGF and Fn,respectively.Protein expressions of total and phosphorylated ERK,FAK(Y397) and p130cas(Y410) were measured by immunoprecipitation and Western blotting.Invasion and proliferation of gastric cancer cells were detected by modified Boyden chamber and MTT assay.RNA interference was used to block FAK expression,and the effect of low level of FAK on cross reaction,cell invasion and proliferation were testified.Results Expressions of total ERK,FAK and p130cas had no significant change before and after stimulation.After stimulation with Fn,tyrosine phosphorylated ERK increased 2.90 times(P0.05);cell invasiveness enhanced 2.36 times(P0.05);and MTT value at 24 h increased 1.68 times(P0.05).After stimulation with EGF,phosphorylated FAK(Y397) increased 2.75 times(P0.05);phosphorylated p130cas(Y410) increased 4.33 times(P0.05);cell invasiveness increased 1.50 times(P0.05);and MTT value at 24 h increased 1.76 times(P0.05).In cells transfected with FAK siRNA,FAK(Y397) phosphorylation was only 0.30 times of the control(P0.05).After stimulation with Fn,ERK tyrosine phosphorylation was only 0.66 times of the control(P0.05);cell invasiveness was only 0.37 of the control(P0.05);and MTT value at 24 h was only 0.63 times of the control(P0.05).After stimulation with EGF,p130(Y410) phosphorylation was only 0.49 times of the control(P0.05);cell invasiveness was only 0.48 times of the control(P0.05);and MTT value at 24 h was only 0.77 times of the control(P0.05).Conclusion There is a crosstalk between EGFR and integrin signaling pathways in human gastric cancer cell SGC7901.FAK,which is a key module in the crosstalk,its inhibition can reduce invasion and proliferation of human gastric cancer cells induced by crosstalk between EGFR and integrin.
【Fund】: 福建省自然科学基金(2009J01148);; 福建省卫生厅创新基金(2009-CXB-14);; 福建医科大学苗圃科研基金(2010MP033)~~
【CateGory Index】: R735.2
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 Zhang Gongyuan1,Guan Wenchi2,Li Shenglei3,Zhang Qiao1,Wu Weidong1(1Department of Epidemiology,Public Health College,2Department of Pathology,Basic Medical College,Zhengzhou University,Zhengzhou,Henan Province,450001;3Department of Clinical Medicine,Medical College of Xiamen University,Xiamen,Fujian Province,361005,China);Silencing nucleostemin in EC9706 cells inhibits tumor growth and expression of EGF and EGFR in xenograft tumor in nude mice[J];Journal of Third Military Medical University;2011-13
2 LI Dan1,DING Jian2,WU Wen-fei 3,YU Jie-ping4,WANG Xiao-zhong1 1.Department of Gastroenterology and Hepatology,The Union Hospital of Fujian Medical University,Fuzhou350001,China;2.Department of Gastroenterology and Hepatology,The First Affiliated Hospital of Fujian Medical University;3.The First Department of Surgery,The Second Hospital of Nanping;4.Department of Gastroenterology and Hepatology,Renmin Hospital of Wuhan University;Effect of Fibronectin-paxillin Pathway on Invasiveness of Human Gastric Cancer Cellline AGS[J];Cancer Research on Prevention and Treatment;2010-09
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 YUE Bao-hong~(1,2),ZHU Xiao-yan~(2),ZHANG Gong-yuan~(2),SUN Ling~(3),ZHAO Xiao-qiang~(3),WANG Qing-xia~(1),LIU Shuai~(1),ZHANG Qiu-tang~(3),ZHANG Qin-xian~(2)(~(1)Clinical Laboratory,~(3)Department of Hematology,The First Affiliated Hospital,~(2)Department of Histology and Embryology,School of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450052,China);Effect of specific short hairpin RNA of nucleostemin on differentiation antigens and biological properties of HL-60 cells[J];Acta Academiae Medicinae Militaris Tertiae;2006-11
2 ZHANG Gong-yuan1, ZHAO Guo-qiang2, YIN Lei1, ZHANG Qin-xian1 1Department of Histology and Embryology, 2Department of Microbiology and Immunology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052, China;Construction and identification of a eukaryotic expression vector for the small interfering RNA targeting nucleostemin gene[J];Journal of Southern Medical University;2008-03
3 YIN Lei1),ZHANG Gongyuan1),LIU Jian1),LI Shenglei2),ZHAO Qiumin2),LIU Zongwen2),CHEN Kuisheng2),ZHANG Qinxian1)1)Department of Histology and Embryology, College of Basic Medical Sciences, Zhengzhou University,Zhengzhou 450001 2)Henan Key Laboratory of Tumor Pathology, Zhengzhou 450052;Expression of EGF protein and mRNA in esophageal squamous cell carcinoma tissue[J];Journal of Zhengzhou University(Medical Sciences);2007-06
4 ZHANG Gong-yuan1,YIN Lei1,LI Sheng-lei2,WU Wei-dong3,CHEN Kui-sheng2,ZHANG Yun-han2,ZHANG Qin-xian 1 (1.Department of Histology and Embryology,Medical College,Zhengzhou University,Zhengzhou 450001,China;2.Department of Pathology,Medical College,Zhengzhou University,Zhengzhou 450001,China;3.Department of Labor Hygiene,Public Health College,Zhengzhou University,Zhengzhou 450001,China);EXPRESSIONS OF NUCLEOSTEMIN,EGF AND EGFR mRNA IN HUMAN ESOPHAGEAL SQUAMOUS CELL CARCINOMA TISSUE[J];Acta Anatomica Sinica;2008-06
5 Alphonse E Sirica, Division of Cellular and Molecular Pathogenesis, Department of Pathology, Virginia Commonwealth University School of Medicine, Richmond, Virginia 23298-0297, United States;Role of ErbB family receptor tyrosine kinases in intrahepatic cholangiocarcinoma[J];世界胃肠病学杂志(英文版);2008-46
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