Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Third Military Medical University》 2012-01
Add to Favorite Get Latest Update

Screening host cell miRNA regulating HBV proliferation and HBsAg expression

Zhang Guangling1,2,Wang Meimei1,Zhu Lihua1,2,Xiong Yanan1,Li Yixuan2,Liu Min2,Tang Hua2,Yuan Lijie1(1Department of Pathogenic Biology,College of Basic Medicine,Hebei United University,Tangshan,Hebei Province,063000;2Tianjin Central Laboratory of Life Sciences,Tianjin Medical University,Tianjin,300070,China)  
Objective To screen out miRNAs that regulate HBsAg expression,HBeAg expression and proliferation of HBV in HepG2 2.2.15 cells,and to provide evidences for interaction between host cell's miRNAs and HBV.Methods Three hundred and twenty eight antisense oligonucleotides of miRNAs were used to inhibit expressions of endogenous miRNAs.Enzyme linked immunosorbent assay(ELISA) and real-time PCR were performed on culture supernatant,which was harvested from HepG2 2.2.15 cells at 72 h after transfection with miRNA ASOs,to detect expressions of HBsAg and HBeAg and copy number of HBV DNA,respectively.Cell proliferation assay(MTS) was used at the same time.Results Three miRNAs,including miR-199a-3pASO,miR-210ASO and miR-185ASO,promoted HBsAg expression and HBV proliferation(P0.05).Overexpression of miR-199a-3p,miR-210 and miR-185 inhibited HBsAg expression and HBV proliferation(P0.05).Compared with those of the control group,HepG2 2.2.15 cells transfected with miR-199a-3pASO,miR-210ASO and miR-185ASO showed a decreased level of miR-199a-3p,miR-210 and miR-185,respectively(P0.05).Cells transfected with miR-199a-3p,miR-210 and miR-185 showed an increased levels of miR-199a-3p,miR-210 and miR-185 compared with those of the control group(P0.05).Conclusion miR-199a-3p,miR-210 and miR-185 inhibit HBsAg expression and HBV proliferation without inhibition of cell proliferation.miRNA,which regulates the interaction between host cell's miRNAs and HBV replication,plays an important role in HBV proliferation.
【Fund】: 天津市自然科学基金(09JCZDJC17500)~~
【CateGory Index】: R512.62
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved