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《Journal of Third Military Medical University》 2012-01
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Curcumin inhibits on proliferation in A549 cells through P53/P21/PCNA/eIF4E signaling pathway

Jiang Jin1,Cao Youde1,Mo Na1,Li Jing1,Mo Xiangang2(1Department of Pathology,Molecular Medicine and Cancer Research Center,College of Basic Medical Sciences,Chongqing Medical University,Chongqing,400016;2Department of Comprehensive Ward,Affiliated Hospital of Guiyang Medical College,Guiyang,Guizhou Province,550004,China)  
Objective To investigate the inhibitory effect of curcumin on the proliferation in A549 cells and to explore possible mechanisms.Methods After A549 cells were treated with different concentrations of curcumin,cell clone formation and proliferation were determined by colony formation assay and MTT assay,respectively.A549 cells were treated with 20 μmol/L curcumin for 24 h,48 h and 72 h,respectively and the cell cycle was measured by flow cytometry(FCM).mRNA and protein levels of P53,P21,PCNA and eIF4E were detected by RT-PCR and Western blotting.Results A549 cells treated with 20 μmol/L curcumin showed an arrest of cell cycle at S phase,inhibition of cell proliferation and reduction of clone formation;the treatment for 24 h resulted in an increased mRNA and protein levels of p53 and p21,which became more obvious after 48 h and 72 h treatment.In addition,the cells treated with 20 μmol/L curcumin for 48 h showed decreased expressions of eIF4E and PCNA at mRNA and protein levels,and the decrease became more obvious after 72 h treatment.Conclusion Curcumin can inhibit clone formation and cell proliferation in A549 cells.The mechanism may be related to up-regulation of P53 and P21 and down-regulation of PCNA and eIF4E.
【CateGory Index】: R285.5
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 Bo ZHANG,Chengchu ZHU Department of Thoracic Surgery,Taizhou Hospital Affiliated to Wenzhou Medical College,Zhejiang 317000,China;Advances of eIF4E Gene and Research in Lung Cancer[J];Chinese Journal of Lung Cancer;2009-08
2 WANG Hai-hui CHENG Yang Institute of Liver Disease,Shanghai University of Traditional Chinese Medicine;Pharmacological Research Progress on Curcumin[J];Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque Shanghai;2007-06
3 LUO Zhi-gang,WANG Yi,BAO Jian-zhong(Department of Pathology,Changhai Hospital,Second Military Medical University,Shanghai 200433,China);Expression and implication of p63,p53 and PCNA in lung cancers[J];Chinese Journal of Clinical Oncology and Rehabilitation;2008-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Xu Lili 1,Limiao2,Du Mingwei1,Chen JiangFan1,Li Jianhua(1Department of Pathology,College of Basic Medical Sciences,China Medical University, Shenyang 110001,China;2Department of Pathology, Liaoning University of Traditional Chinese Medicine;Shenyang 110034,China);Molecular biological observation and study of lung cancer cells from sputum[J];Chinese Journal of Histochemistry and Cytochemistry;2011-06
2 ZHANG Qiong~1,LI Lin~2,WEI Dong-zhi~1,YANG Sheng-li~1(1.School of Biotechnology,East China University of Science and Technology,Shanghai 200237,China;2.Unilever Research Center,Shanghai 200233,China);Solubilization Properties of Hydrophobic Curcumin by Bile Salt/Lipid Mixed Micelles[J];Journal of East China University of Science and Technology(Natural Science Edition);2010-05
3 SUN Yong1,WANG Yun1,ZHOU Jing2,FANG Tai-hui2,XU Bin1(1.Key Laboratory of Integrative Acupuncture and Pharmacology,Nanjing University of Chinese Medicine,Nanjing,210046,China;2.School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing,210046,China);Exploration on the Method of Determining Curcumin Pharmacokinetics by in-vivo Imaging[J];Journal of Nanjing University of Traditional Chinese Medicine;2011-06
4 ZHANG Wei-min, XU Ji-ping, HUANG Wen. (Department of Gastroenterology, No.421 Hospital of PLA, Guangzhou 510530, Guangdong, P. R.China );The effects of curcumin on cellular cycle of pancreatic carcinoma cells in vitro[J];China Tropical Medicine;2008-12
5 FAN Tao1,GAO Su-ya1,2,WANG Li1,YANG Li-ning1,LI Hua2(1.Department of Pharmaceutical Science,Xi'an Medical University,Xi'an 710021,China; 2.Institute of Analytical Science,Northwest University,Xi'an 710069,China);Synthesis of curcumin-cerium coordination and its antioxidation characteristics[J];Applied Chemical Industry;2011-03
6 HAN Gang1,WAN Hong2,ZHAI Guan-yu1,FAN Ying1(1.Pharmaceutical Department of North China Coal Medical University,Tangshan 063000,China;2.North China Coal Medical University,Qinhuangdao Branch College,Qinhuangdao 066000,China);Protective Effect of Curcumin Solid Dispersion on Acute Liver Injury Induced by Carbon Tetrachloride in Mice[J];Lishizhen Medicine and Materia Medica Research;2009-06
7 Gang Han, Hai-Zhong Yuan, Yue Dong, Pharmaceutical Science, North China Coal Medical University, Tangshan 063000, Hebei Province, China Guo-Xian Yao, Department of Pharmacy, Fengrun People’s Hospital Tangshan 064000, Hebei Province, China;Effects of curcumin solid dispersion on oxidative stress of alcoholic hepatic injury in rats[J];World Chinese Journal of Digestology;2009-05
8 CHEN Li-hua,XIAO Xin-yu,LI Zi-bo,CHENG Yang-yan Department of Clinical Laboratory Medicine,Changsha Medical University,Changsha 410219,Hunan,China;Protective effect of curcumin on Con A-induced liver injury in mice and its mechanism[J];Chinese Journal of Clinical Pharmacology and Therapeutics;2010-09
9 ZHANG Zhen-jun,LIU Zheng,WAN Liang-gang.Department of Thoracic Surgery,Handan Center Hospital Hebei,Handan 056001,China;Effects of curcumin on SK cell proliferation and expression of PCNA,P53 mRNA in ovary cancer[J];Chinese Journal of Difficult and Complicated Cases;2009-08
10 SI Hong,XU Yan-yan(Jiangsu Nantong Tumor Hospital,Nantong 226361,China);Effects of Curcumine on the Proliferation of Gastric Cancer Cell Line SGC-7901 and Expressions of COX-2mRNA and COX-2 Protein[J];Evaluation and Analysis of Drug-Use in Hospitals of China;2010-12
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 Cheng Yang,Ping Jian,Xu Lieming,et al.Institute of Liver Diseases,Shuguang Hospital Affiliated to Shanghai University of TCM,Shanghai 201203,China;Curcumin inhibits the activation marker of hepatic stellate cells by up-regulating the peroxisome proliferator-activated receptorγ.[J];Chinese Journal of Practical Internal Medicine;2006-24
2 CHEN Hua, XUE Chang-gao, CHEN Tie-hui, WANG Jia-li, SUN Chang-sheng (Toxicological Laboratory of Chinese Medicine, School of Public Health, Fujian Medical University, Fuzhou 350004,China);Chemopreventive effect of curcuma and curcumin on liver injury induced by microcystins in mice[J];Chinese Pharmacological Bulletin;2005-12
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