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《Journal of Third Military Medical University》 2012-01
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Curcumin inhibits on proliferation in A549 cells through P53/P21/PCNA/eIF4E signaling pathway

Jiang Jin1,Cao Youde1,Mo Na1,Li Jing1,Mo Xiangang2(1Department of Pathology,Molecular Medicine and Cancer Research Center,College of Basic Medical Sciences,Chongqing Medical University,Chongqing,400016;2Department of Comprehensive Ward,Affiliated Hospital of Guiyang Medical College,Guiyang,Guizhou Province,550004,China)  
Objective To investigate the inhibitory effect of curcumin on the proliferation in A549 cells and to explore possible mechanisms.Methods After A549 cells were treated with different concentrations of curcumin,cell clone formation and proliferation were determined by colony formation assay and MTT assay,respectively.A549 cells were treated with 20 μmol/L curcumin for 24 h,48 h and 72 h,respectively and the cell cycle was measured by flow cytometry(FCM).mRNA and protein levels of P53,P21,PCNA and eIF4E were detected by RT-PCR and Western blotting.Results A549 cells treated with 20 μmol/L curcumin showed an arrest of cell cycle at S phase,inhibition of cell proliferation and reduction of clone formation;the treatment for 24 h resulted in an increased mRNA and protein levels of p53 and p21,which became more obvious after 48 h and 72 h treatment.In addition,the cells treated with 20 μmol/L curcumin for 48 h showed decreased expressions of eIF4E and PCNA at mRNA and protein levels,and the decrease became more obvious after 72 h treatment.Conclusion Curcumin can inhibit clone formation and cell proliferation in A549 cells.The mechanism may be related to up-regulation of P53 and P21 and down-regulation of PCNA and eIF4E.
【CateGory Index】: R285.5
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