Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Third Military Medical University》 2012-01
Add to Favorite Get Latest Update

Dynamic expression of acetylated histone in transcription regulation area of fatty acid synthase related to liver X receptor

Yu Huihong,Shen Wei,Guo Jinjun(Department of Gastroenterology,Second Affiliated Hospital,Chongqing Medical University,Chongqing,400010,China)  
Objective To determine the expression of acetylated histone in transcription regulation area of fatty acid synthase(FAS) gene with liver X receptor(LXR) agonist T0901317 and whether acetylated histone induces chromatin remodeling during expression of FAS gene transcription.Methods Transcription expression time spectrum of FAS mRNA was detected by real-time fluorescence quantitative PCR.H3 and H4 expression levels at 4 transcription regulation area sites of FAS gene were measured by chromatin immunoprecipitation and real-time fluorescence quantitative PCR respectively.Results The expression level of FAS mRNA increased in a time-dependent manner in 2 h after T0901317 was used(1.09,P0.05) and reached its peak at 16 h(4.25,P0.01),which was significantly higher at 48 h than at 0 h(P0.01).The expression level of acetylated H3 was significantly higher at 30 min than at 0 h after T0901317 was used(P0.05),and was lower than that at 0 h with a difference at 2 000 bp(P0.05) and close to or higher at 2 h than at 0 h with a difference at LXRE(P0.05).The expression level of acetylated H4 was also higher at 30 min than at 0 h after T0901317 was used(P0.05) and lower at 1 and 2 h than at 0 h(P0.05).Conclusion The expression level of FAS mRNA is increased in a time-dependent manner when the T0901317 is used.Acetylated histone at 2 500 bp upstream and downstream at the transcription start site during early FAS transcription induces chromatin remodeling with the site of transcription regulation area,the time when the T0901317 is used,and the expression level of histone.
【Fund】: 国家自然科学基金(30871160)~~
【CateGory Index】: R575.5
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 1 Hits
1 WANG Yan 1, GUO Qiao-nan 2, ZHANG Bo 1, XU Xue-qing 1, BAI Yun 1 ( 1Department of Medical Genetics, College of Medicine, 2Institute of Pathology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Regulation of histone acetylation on cell cycle and p53,p21~(WAF1) genes expression in malignant transformation of IEC-6[J];Acta Academiae Medicinae Militaris Tertiae;2007-04
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 Sun Yan1,2,Peng Qiongle1,2,Wang Liyang1,2,Zhao Liuyang1,2,Yuan Quan1,Li Jun1,Liu Manran1,2(1Key Laboratory of Diagnostic Medicine of Ministry of Education,2Department of Cell Biology and Genetics,College of Basic Medical Sciences,Chongqing Medical University,Chongqing,400016,China);Biological characteristics of fibroblasts primarily cultured form breast precancerosis[J];Journal of Third Military Medical University;2012-01
2 Shi Jiaojiao1,Mi Mantian2,Wei Na2,Wang Bin2 1.Department of Trophology,General Hospital of Chengdu Military Command,Chengdu,Sichuan,610083,China;2.Department of Nutrition and Food Hygiene/Chongqing Key Laboratory of Nutrition and Food Safety,Third Military Medical University,Chongqing,400038,China;Effects of different dietary fatty acid composition on HMG-CoAR and SREBP-1c expression in rat liver[J];Medical Journal of National Defending Forces in Southwest China;2011-08
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 WANG Yan 1 , GUO Qiao-nan 2 , ZHANG Bo 1 , LIU Xin 1 , BAI Yun 1 ( 1 Department of Molecular Genetics, College of Medicine, 2 Department of Pathology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Ability of malignant transformation of rat IEC-6 by MNNG and PMA[J];Acta Academiae Medicinae Militaris Tertiae;2006-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved