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《Journal of Third Military Medical University》 2012-01
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Relationship of gastric motility with vasculopathy in rats at early stage of type 2 diabetes mellitus

Wang Qiong1,Yan Xiang1,Li Weixin1,Gao Chengxia1,Sun Xiaowei2(1Department of Gerontology,First Affiliated Hospital,2Department of Pathology,College of Preclinical Medicine,LanZhou University,Lanzhou,Gansu Province,730030,China)  
Objective To observe the changes of nitric oxide,endothelial nitric oxide synthase(eNOS) in gastric antrum tissue and soluble endothelial cell protein C receptor(sEPCR) in the serum in the diabetic rats,and to investigate the relationship of the gastric motility change and gastric vasculopathy in the early stages of type 2 diabetes mellitus.Methods A total of 25 male SPF SD rats were first divided random in two groups: control group(NC,n=10) and diabetic mellitus group(DM,n=15).The rats in the DM group were fed with fat-and caloric-rich diet and induced by intraperitoneal injection of streptozotocin(STZ).After the induction,13 rats were finally identified as DM rats.At 12 weeks,gastric emptying(GET1/2) and gastric emptying rate were measured in NC groups and DM groups by single photo emission computed tomography(SPECT).Enzyme-linked immunosorbent assay was employed to examine the serum level of sEPCR.NO in gastric antrum tissue was detected by nitrate reductase,and eNOS observation in the gastric tissue was measured by immunohistochemical assay.Results The gastric emptying,gastric NO content,and mean density of eNOS were significantly lower in DM group(30.82±12.56 min,30.82±12.56 μmol/L and 0.36±0.15,respectively) than in NC group(P0.05).While the serum level of sEPCR was significantly higher in DM group(129.45±4.83 μg/L) than in NC group(P0.01).Conclusion Our results indicate that gastric emptying is accelerated in the early stages of DM.In this stage,vascular endothelium is impaired,and thus results in gastric motility disorders.
【Fund】: 甘肃省卫生行业科研计划项目(GSWST09-04);; 兰州大学中央高校基本科研业务费专项资金(lzujbky-2009-148)~~
【CateGory Index】: R587.2
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