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《Journal of Third Military Medical University》 2012-01
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Effect of different dietary fatty acids on expression of sterol-regulatory elementary binding protein-1c and lipid metabolism in hepatic cells

Tian Xiaoyuan,Zhang Qianyong,Ran Li,Mi Mantian(Department of Nutrition and Food Hygiene,Chongqing Medical Nutrition Research Center,Chongqing Key Laboratory of Nutrition and Food Safety,College of Military Preventive Medicine,Third Military Medical University,Chongqing,400038,China)  
Objective To study the effect of different dietary fatty acid compositions on expression of sterol-regulatory elementary binding protein-1c(SREBP-1c) and lipid metabolism in hepatic cells.Methods HepG2 cells were divided into control group,eicosapntemacnioc acid(EPA) group,palmitic acid(PA) group,1∶1 EPA and linoleic acid(LA) mixture group,1∶1 PA and oleic acid(OA) mixture group.The final concentration of EPA and LA was 150 μmol/L in EPA group and PA group respectively,and 75 μmol/L in EPA and LA mixture group and PA and OA mixture group respectively.The total concentration was 150 μmol/L 24 h after the HepG2 cells were treated.Changes of lipid droplet and its level in HepG2 cells were observed with oil red staining.Expression level of SREBP-1c mRNA and protein was measured by RT-PCR and Western blotting respectively.Results The expression level of SREBP-1c mRNA and protein was significantly higher in PA group and 1∶1 PA and OA mixture group,and significantly lower in EPA group and 1∶1 EPA and LA mixture group than in control group(P0.05).The lipid level in hepatic cells was significantly lower in EPA group and 1∶1 EPA and LA mixture group than in control group(P0.05),and significantly higher in PA group and 1∶1 PA and OA mixture group(P0.05).Conclusion PA and OA up-regulate the expression of SREBP-1c and promote the synthesis of lipid by promoting the fatty degeneration in hepatic cells,while EPA and LA down-regulate the expression of SREBP-1c and decrease the lipid level by reducing the fatty degeneration in hepatic cells.
【Fund】: 国家自然科学基金(30972469)~~
【CateGory Index】: R575.5
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1 Madhusudana Girija Sanal, Special Center for Molecular Medicine, Jawaharlal Nehru University, New Delhi 110067, India;The blind men 'see' the elephant-the many faces of fatty liver disease[J];世界胃肠病学杂志(英文版);2008-06
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1 TIAN Xiao-yuan,ZHANG Qian-yong,Ran Li,(Department Of Nutrition and food Hygiene,College of Preventive Medicine,Third Military Medical University,Chong qing Research Center Of Nutrition and food safety,Chong qing 400038,China);Effects of the different dietary fatty acid composition on expression of SREBP-1c mRNA and lipid metabolism in cultured hepatocytes[A];[C];2011
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