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《Journal of Third Military Medical University》 2012-01
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Effect of high glucose concentration on expression of toll-like receptor-2 in ARPE-19 cells

Chen Xuemei,Zhang Xuedong,Zong Yuanjuan,Shen Qiang(Department of Ophthalmology,Chongqing Key Laboratory of Ophthalmology,First Affiliated Hospital,Chongqing Medical University,Chongqing,400016,China)  
Objective To observe the effect of different glucose concentrations on expression of toll-like receptor-2(TLR-2) in ARPE-19 cells.Methods ARPE-19 cells were divided into 5.5 mmol/L glucose group,15.5 mmol/L glucose group,25.5 mmol/L glucose,25.5 mmol/L glucose +10 mmol/L Go6976 PKC-α inhibition group,5.5 mmol/L glucose + 20 mmol/L mannitol control group.Expression level of TLR-2 in ARPE-19 cells and translocation of PKC-α from cytoplasm to cytomembrane were measured by Western blotting,while that of TLR-2 mRNA in ARPE-19 cells was measured by real-time PCR.Results The expression level of TLR-2 mRNA and the translocation of PKC-α from cytoplasm to cytomembrane were higher in 25.5 mmol/L glucose group and 15.5 mmol/L glucose group than in control group(P0.05).The expression level of TLR-2 protein and the translocation of PKC-α from cytoplasm to cytomembrane were significantly lower in PKC-α inhibitor inhalation group than in 25.5 mmol/L glucose group(P0.01).Conclusion High glucose concentration can up-regulate the expression of TLR2 mRNA in ARPE-19 cells and promote the translocation of PKC-α from cytoplasm to cytomembrane through the PKC-α pathway.
【Fund】: 重庆市卫生局重点项目(2011-1-029)~~
【CateGory Index】: R587.2
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【Citations】
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1 DAI Chao, BIAN Xiu-wu, SHI Jing-quan (Department of Pathology, Southwest Hospital, Third Military Medical University, Chongqing 400038,China);Nordy inhibits the expression of vascular endothelial growth factor and inducible nitric oxide synthase in diabetic rat retinas[J];Acta Academiae Medicinae Militaris Tertiae;2005-12
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