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《Journal of Third Military Medical University》 2012-19
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Effect of sinomenine on IL-8 expression in porphyromonas gingivalis lipopolysaccharide-stimulated human gingival fibroblasts

Wang Chun,Xiang Xuerong,Yang Mingcong,Chen Fangchun(Department of Periodontology,Hospital of Stomatology,Chongqing Medical University,Chongqing,401147,China)  
Objective To study the effect of sinomenine(Sin) on IL-8 expression in porphyromonas gingivalis lipopolysaccharide(LPS)-stimulated human gingival fibroblasts(HGF).Methods HGF were pretreated with Sin at the concentrations of 25,50 and 100 μmol/L for 24 h and then with 1 μg/ml LPS which was added into Sin.Effect of Sin on LPS-mediated IL-8 expression in HGF was detected by RT-PCR and ELISA,respectively.Effect of Sin on NF-κB translocation and IκB degradation was detected by Western blotting.Effect of NF-κB inhibitors,Bay11-7085(10 μmol/L) and TPCK(20 μmol/L),on Sin-mediated IL-8 expression in HGF,was detected by ELISA 24 h after pretreatment followed by 4 h-LPS stimulation.Results Sin suppressed the IL-8 mRNA and protein expressions in LPS-mediated HGF in dose-dependent manner(P0.05).SIN could almost keep the NF-κB in the cytoplasm compared with LPS.Most NF-κB was transported in the nuclei 1 h after LPS treatment.The NF-κB level was 2-fold higher than the LPS level in cytoplasm after Sin(100 μmol/L) pretreatment.Fifty percent of IκBα was degraded 1 h after the HGF were pretreated with LPS and Sin inhibited the LPS-mediated degradation of IκBα in a dose-dependent manner after HGF were pretreated(P0.05).Sin(50 μmol/L) pretreatment returned the LPS-mediated IκBα degradation to its normal levels.The IL-8 expression level was 2.4-fold higher in LPS pretreatment group than in control group,and 1.8-fold and 1.6-fold lower in LPS pretreatment group than in control group after pretreatment with NF-κB inhibitors,Bay11-7085(10 μmol/L) and TPCK(20 μmol/L).Conclusion Sin inhibits the porphyromonas gingivalis LPS-mediated IL-8 expression in HGF by blocking the NF-κB translocation and the IκBα degradation.
【CateGory Index】: R285.5
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