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《Journal of Third Military Medical University》 2018-01
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Relationship of mitochondrial fusion/fission genes with dihydromyricetin inhibiting liver fat accumulation in high-fat fed mice

MA Xin;CHEN Ka;RAN Li;ZHU Jundong;MI Mantian;Research Center of Nutrition and Food Safety,Chongqing Key Laboratory of Nutrition and Food Safety,Chongqing Center of Medical Nutrition,Institute of Military Preventive Medicine,Third Military Medical University;Department of Rehabilitation Medicine,General Hospital of Chengdu Military Command;  
Objective To investigate the role of the hepatic mitochondrial fusion/fission-related genes in the dihydromyricetin(DHM)-induced inhibition of liver fat accumulation in mice after high-fat diet(HFD). Methods Forty-five male C57 BL/6 J mice(6 weeks old) were randomly divided into control group(CON),HFD group(HFD) and HFD with DHM treatment(HFD + DHM),with 15 mice per group. After 12 weeks,the body weight,hepatic index and serum biochemical indexes were detected. HE and oil red O staining were applied to observe the changes of hepatic steatosis and lipid droplets accumulation. The ATP production was measured by an enhanced ATP detecting kit. The mRNA and protein levels of mitochondrial fusion/fission genes,fission 1(Fis1),dynamin-related protein 1(Drp1),mitofusion 2(Mfn2) and optic atrophy1(Opa1) were determined by PCR and Western blotting. Results HFD significantly increased body weight,hepatic index, fasting glucose, fasting insulin, triglyceride, total cholesterol and low density lipoprotein(P 0. 05),while decreased the ATP production(P 0. 05). HE staining displayed notable intrahepatic fat vacuoles and hepatocyte balloon-like changes in the liver of HFD mice. And oil red O staining showed more fat droplets in HFD mice. The mRNA and protein levels of hepatic Fis1 and Drp1 were significantly increased(P 0. 05),while those of Mfn2 and Opa1 were dominantly decreased in HFD group(P 0. 05). However,DHM treatment could significantly reverse all above changes induced by HFD(P 0. 05). Conclusion DHM attenuates lipid accumulation in the HFD mice,which may be related to its regulation on mitochondrial fusion/fission functions.
【Fund】: 国家自然科学基金面上项目(81372975)~~
【CateGory Index】: R575
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