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《Journal of Third Military Medical University》 2018-01
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Expression profile of AQP9 in liver of rats with type 2 diabetes mellitus

LI Yuan;HOU Jiaojiao;REN Xuxia;CHEN Yuqin;YI Yaoxing;YANG Mei;Neuroscience Center,Chongqing Medical University;First Clinical College of Chongqing Medical University;  
Objective To investigate the expression profile of aquaporin-9(AQP9) in the liver of type 2 diabetes mellitus(T2 DM) rats and explore the possible regulatory mechanism. Methods Male adult Sprague-Dawley rats were fed with high fat diet and given an intraperitoneal injection of streptozotocin(STZ)to induce T2 DM model. Hematoxylin-eosin(HE) staining was used to observe the morphological changes in the liver. Immunofluorescence assay was employed to investigate the distribution of AQP9 in the liver. Western blotting was adopted to detect the hepatic expression of AQP9 at protein level. Results Compared with the control group, the hepatic cells in the T2 DM rats were swollen, with hydropic degeneration, and in disorganized arrangement,and some inflammatory cells had infiltrated in the liver tissue. Immunofluorescent microscopy showed that AQP9 was rarely expressed in the liver of control rats,but massively expressed in the liver of T2 DM rats,mainly concentrating in rings in the cell membrane towards hepatic sinusoid. Western blotting demonstrated that the expression level of AQP9 was significantly increased(P 0. 05),and the ratio of p-p38 to p38 was obviously reduced(P 0. 05),and that of p-JNK to JNK notably increased(P 0. 05),while that of p-ERK to ERK had no change(P 0. 05). Conclusion The expression of AQP9 is obviously increased in the liver of T2 DM rats. p38-MAPK and JNK-MAPK signal pathways may play regulatory role in its expression in hyperglycemic condition.
【Fund】: 国家自然科学基金面上项目(81671312 81000566);; 重庆市自然科学基金(CSTC2015jcyA10018);; 重庆医科大学基础医学院苗圃基金(JC201506);重庆医科大学大学生科学研究与创新实验(201502)~~
【CateGory Index】: R575;R587.2
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