GP73 regulates proliferation and apoptosis of breast cancer MCF-7 via p53 signaling pathway
SHUI Zhengrong;TANG Xueyuan;Department of Oncology,the Third Xiangya Hospital,Central South University;
Objective To determine the impact of Golgi protein 73(GP73) on the proliferation and apoptosis of breast cancer MCF-7 cells and investigate its related mechanism. Methods After MCF-7 cells were separately transfected with GP73 shRNA and overexpression plasmids,CCK-8 assay and flow cytometry were employed to detect the proliferation and apoptosis,respectively in the transfected cells. The expression of GP73 and p53 at mRNA and protein levels was determined by qRT-PCR and Western blotting. The mRNA expression of above 2 molecules was detected in 20 breast cancer tissues and paired paracancerous tissues with qRT-PCR. The correlation of GP73 and p53 mRNA levels in breast cancer tissue were analyzed. Results GP73 overexpression enhanced the proliferation and suppressed the apoptosis of MCF-7 cells,while GP73 down-regulation inhibited the proliferation and improved apoptosis of MCF-7 cells. Further,co-overexpression of GP73 and p53 resulted in reduced proliferation and increased apoptosis when compared with the MCF-7 cells overexpressed with GP7 or p53 alone. A negative correlation was observed between the mRNA expression levels of GP73 and p53 in breast cancer tissues. Conclusion GP73 may regulate proliferation and apoptosis of breast cancer MCF-7 cells via p53 signaling pathway.
【CateGory Index】： R737.9