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《Journal of Third Military Medical University》 2018-06
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Silencing calcium-activated nucleotidase 1 inhibits proliferation and migration of clear cell renal cell carcinoma 769-P cells

LIU Xin;LIU Xiaobing;LIU Qian;WANG Qingqing;YANG Zhenxing;LI Longkun;Department of Urology,Second Affiliated Hospital,Army Medical University (Third Military Medical University);  
Objective To determine the effect of calcium-activated nucleotidase 1( CANT1) on the proliferation and migration in human clear cell renal cell carcinoma 769-P cells,and preliminarily investigate its underlying mechanism. Methods The expression of CANT1 was detected by immunohistochemistry in the intra-operatively obtained tumor tissues and paracancerous normal tissues. Lentiviral vectors carrying shRNA interfering CANT1 and blank control shRNA were used to infect the 769-P cells,then RT-PCR and Western blotting were adopted to verify the interference efficiency at mRNA and protein levels. After stable transfection,cell proliferation,migration,cell cycle distribution and apoptosis were measured. Results The 769-P cells with stable CANT1 knockdown were successfully established. Immunohistochemistry showed that CANT1 was expressed only in the distant renal tubules of normal renal tissues,but was with little or no expression in the proximal renal tubule. In the tissues of clear cell renal cell carcinoma,the protein was intensively expressed. CANT1 knockdown significantly inhibited the cell proliferation( P 0. 01) and cell migration by wound healing assay( P 0. 01),arrested the cells at S phase,and enhanced cell apoptosis in the 769-P cells after transfection with lentiviral vectors carrying shRNA CANT1 than those transfected with blank control shRNA( P 0. 05). Conclusion CANT1 knockdown can significantly inhibit the proliferation and migration,arrest cell cycle at S stage and promote the apoptosis in clear cell renal cell carcinoma 769-P cells.
【Fund】: 国家自然科学基金面上项目(81470989)~~
【CateGory Index】: R737.11
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