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《Acta Zootaxonomica Sinica》 2007-03
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COMPARISON OF CYT B AND COⅠ GENE SEQUENCES FROM 15 SPECIES IN PASSERIFORMES

LIANG Gang1, ZHANG Wei 1,2 , LEI Fu-Min2, YIN Zuo-Hua2, HUANG Yuan1, LI Tian-Xian31. College of Life Sciences, Shaanxi Normal University, Xi'an 710062, China2. Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China3. Wuhan Institute of Uivology, Chinese Academy of Sciences, Wuhan 430071, China  
By using two molecular markers of mitochondrial DNA, i.e. Cyt b and CoⅠ gene, the sequencess of 15 species representing 6 families from Passeriformes were compared. The results showed that the variable sites of Cyt b and CoⅠ gene sequence were 454 and 366, and parsimony information sites were 337 and 303 respectively. Moreover, CoⅠ gene was more conservative and had a lower evolutionary rate than Cyt b gene. Neighbor-joining (NJ), maximum-parsimony (MP), maximum-likelihood (ML) and Bayesian (BI) analyses generated nearly identical tree topologies and their fifty percent consensus trees were computed. The results of these phylogenetic trees were compared and analyzed, too. It was also revealed that CoⅠ gene was more suitable to identify the phylogenetic relationship of avian family unit than Cyt b gene, and CoⅠ gene can be a molecular marker to identify avian species. But in species identification, CoⅠ gene was less stable and accurate than Cyt b gene.
【Fund】: 国家自然科学基金资助项目(30670276 30270203 30599431);; 中国科学院动物研究所知识创新领域前沿项目(KSCX3-IOZ-0614);; NSFC/KOSEF协议项目(30611140611);; 陕西师范大学校级重点科研项目资助
【CateGory Index】: Q953
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