Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Cryogenics》 2006-01
Add to Favorite Get Latest Update

Biological chip technology for quickly batch selecting optimum cryopreservation procedure

Yu Lina~1 Liu Jing~1 Zhou Yixin~1 Hua Zezhao~2(~1 Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Beijing 100080,China)(~2 School of Power Engineering,Shanghai University for Science and Technology,Shanghai 200093,China)  
In the practices of cryobiology,selection of an optimum freeze/thawing program and idealistic cryo-protective agent often requests rather tedious,time consuming and repetitive tests.Integrating the functions of sample preparation and viability detection,the concept of biochip technology to the field of cryopreservation,aiming to quickly find out an optimum freezing and thawing program is introuduced.The prototype devices were fabricated and the corresponding experimental tests were performed.It was shown that microflow-channel chip could not realize a high quality solution distribution.As an alternative,the spot-dropping chip provides an excellent way to load the sample quickly and reliably.Infrared thermal mapping on such a chip shows that it has a rather uniform heat transfer boundary.Applying the spot-dropping chip combined with the semiconductor cooling device,the final output of cryopreservation of multiple samples was tested,and the optimal freeze/thawing program as well as the potentially best concentration of the cryo-protective agent was found out by analyzing the results.The application of this technique to measure the thermo-physical properties of the cryo-protective agent was investigated.This study demonstrates that the biochip with integrated automatic loading and the inspection units can make it possible for massive optimization on the complex cryopreservation program to be a much quick and economic way.
【Fund】: 国家自然科学基金(50575219及50436030)资助项目
【CateGory Index】: TB611
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 TIAN Yong Sheng ①② CHEN Song Lin ②** YAN An Sheng ① JI Xiang Shan ② YU Guo Cai ② (① Huazhong Agricultural University, Wuhan 430070, China) (② Yeallow Sea Fishery Research Institute,CAFS, Key Lab For Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Qingdao 266071, Shandong, China);Cryopreservation of the sea perch (Lateolabrax japonicus) embryos by vitrification[J];Acta Zoologica Sinica;2003-06
2 JIANG Yan1,HAO Wen-li2,TIAN Ye1(1.Henan Science and Technology University,Luoyang,Henan 471003,China;2.Farm of Jun County,Hebi,Henan 458030,China);Influence on Physiological and Biochemical Characteristics of Pumpkin Seeds after Cryopreservation Abstract[J];Northern Horticulture;2009-02
3 Liu Yan; Gao Rongfu; Zhou Xiaoyang.College of Landscape Architecture, Beijing For. Univ., 100083, P.R. China.;Changes of ATPase in plasma membrane during cryopreservation by vitrification with seedlings of Arabidopsis thaliana.[J];Journal of Beijing Forestry University;2001-05
4 SHU Zhiquan1,LUO Zhaofeng2,GUO Xiaojie3,LIU Zhong3,HE Liqun1,and GAO Dayong 1,4.1 Department of Thermal Science and Energy Engineering,University of Science and Technology of China,Hefei 230027;2 School of Life Science,University of Science and Technology of China,Hefei 230027;3 The Red Cross Transfusion Center of Hefei,Hefei 230022;4 Department of Mechanical Engineering,University of Washington,Seattle,USA.;The Experimental Study of Osmotic Injury to Erythrocytes in Freeze Drying[J];Beijing Biomedical Engineering;2006-06
5 CHENG Bo,YE Ming-fu (Department of Cardiothoracic Surgery,Xingqiao Hospital, the Third Military Medical University, Chongqing 400037,China);The effect and complications of cryosurgery on adjacent organs of lung and residual bronchia[J];Chongqing Medical Journal;2003-02
6 HU Hui-hong1,QIAN Bao-hua1,LIU Bao-lin2,GU Hai-hui1,ZHU Yan-xia1,HUA Mei-xian1 1.Department of Blood Transfusion,Changhai Hospital,Second Military Medical University,Shanghai 200433,China2.Biotechnology Institute of Low Temperature,University of Shanghai for Science and Technology,Shanghai 200093,China;Influence of cooling rate on quality of cryopreservted platelets[J];Academic Journal of Second Military Medical University;2008-07
7 FAN Wen-xia1,MA Xue-hu1,YU Xiao-chuan1,LIU Tian-qing1,CUI Zhan-feng21.School of Chemical Engineering,Dalian University of Technology,Dalian 116024,China;2.Department of Engineering Science,Oxford University,Oxford OX1 3PJ,UK;Cryopreservation of in vitro model of corneal endothelia by vitrification[J];Journal of Dalian University of Technology;2008-06
8 Chen Zhenqian Chen Yu Shi Mingheng (School of Energy and Environment,Southeast University,Nanjing 210096,China);Numerical simulation of freezing phase change process in biological tissue[J];Journal of Southeast University(Natural Science Edition);2007-01
9 LIU Ying-Yan, ZHONG Li-Sheng, XU Xiao Zhou,MAO Tian-Qiu,XU Chuan-Xiang Electrical Insulation Institute, Xi'an Jiaotong University, Xi'an 710049 Department of Maxillofacia Surgery, College of Stomatology, Fourth Military Medical University;Experimental research on the low-temperature dielectric properties of tumor tissue[J];Journal of The Fourth Military Medical University;1998-02
10 GU Ai--Mei;DONG Zhao-Shen;DENG Xin-Zhu;(Department of Military Hygiene,Faculty of MilitaryHealth Service and statistics,Fourth Military Medical University,Xi'an 710033, China);Influence of exogenous metallothionein gene on coldtolerance of mouse fibroblasts[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;1999-01
China Proceedings of conference Full-text Database 10 Hits
1 YANG Rui-sen, LIU Qi, LI Min, ZHANG Bai-Jiang, ZHANG Li-min, LI Hui, LIU Xi-bin, YANG Jing. Department of Thoracic Surgery , Shandong tumor Hospital & Institute , Jinan 250117, China;Clinical studies on endoscopy-guided intracavity cryotherapy of esophagus with flexible cryoprobes via soft rube[A];[C];2006
2 Wu Zheng-zhen;Hua Ze-zhao;Liu Bao-lin;He Hui;Li Chuan;Chen Ying Institute of Cryogenic Engineering,University of Shanghai for Science and Technology, Shanghai 200093 Shanghai Antu Hospital,Shanghai 200093;Experimental Study of Glycerol on Freeze-dried Human Red Blood Cells[A];Chinese association of Refrigeration[C];2005
3 Liu Baolin Wang Xin Hua Zezhao Insdtute of Cryobiology, Shanghai University of Science and Technology, 200093, Shanghai;Effect of Cryoprotective Agents on the Morphology and Viability of Attached Osteoblast Cells[A];Chinese association of Refrigeration[C];2006
4 HE Hui, LIU Bao-Lin, HUA Ze-Zhao, LI Chuan, SHEN Min, CHEN Ying Shanghai University of Science and Technology, China, 200093. Institute of Shanghai Antu Hospital Blood Test, China, 200093.;Effects of Residual Water Moisture on Red Blood Cells by Freeze-drying[A];Chinese association of Refrigeration[C];2006
5 Huiling Wang Suo Li Chenfeng Wang Department of Refrigeration and Cryogenics Huazhong University of Science and Technology, Wuhan 430074;The numerical simulation of the temperature field of bio-tissue in cryosurgery[A];Chinese association of Refrigeration[C];2006
6 Chenfeng Wang Decun Xu Huiling Wang Department of Refrigeration and Cryogenics Huazhong University of Science and Technology, Wuhan 430074 * 8 th Hospital of Wuhan;Characteristics and applications of a compact cryosurgical instrument with liquid nitrogen transport by soft pipe[A];Chinese association of Refrigeration[C];2006
7 Gao Zhixin,Hao Baotong,Liu Baolin,Rong Senjie,Wang Xin,Zhou Guoyan Institute of Biothermal and Technology,University of Shanghai for Science and technology,shanghai 200093;A Review on the Cryopreservation of Arteries[A];Chinese association of Refrigeration[C];2009
8 ;Analyze on the phase transformation point of small molecule carbohydrate loaded platelet+ anti-freeze dried reagent muddled solution[A];Chinese association of Refrigeration[C];2009
9 WANG bo-chun,LIU bao-lin,LI dai-xi,YU de-yang Biology system thermal science lab,university of Shanghai for science and technology,200093,China;Simulations of vitrifying processes for water under different conditions[A];Chinese association of Refrigeration[C];2009
10 Hao Baotong,Liu Baolin,Wang Xin,Deng Wanzi Institute of Biothermal Science,University of Shanghai for Science and Technology,shanghai,200093;The toxicity of Cryoprotective agents on Attached Osteoblast cells[A];Chinese association of Refrigeration[C];2009
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved