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《Cryogenics》 2006-01
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Biological chip technology for quickly batch selecting optimum cryopreservation procedure

Yu Lina~1 Liu Jing~1 Zhou Yixin~1 Hua Zezhao~2(~1 Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Beijing 100080,China)(~2 School of Power Engineering,Shanghai University for Science and Technology,Shanghai 200093,China)  
In the practices of cryobiology,selection of an optimum freeze/thawing program and idealistic cryo-protective agent often requests rather tedious,time consuming and repetitive tests.Integrating the functions of sample preparation and viability detection,the concept of biochip technology to the field of cryopreservation,aiming to quickly find out an optimum freezing and thawing program is introuduced.The prototype devices were fabricated and the corresponding experimental tests were performed.It was shown that microflow-channel chip could not realize a high quality solution distribution.As an alternative,the spot-dropping chip provides an excellent way to load the sample quickly and reliably.Infrared thermal mapping on such a chip shows that it has a rather uniform heat transfer boundary.Applying the spot-dropping chip combined with the semiconductor cooling device,the final output of cryopreservation of multiple samples was tested,and the optimal freeze/thawing program as well as the potentially best concentration of the cryo-protective agent was found out by analyzing the results.The application of this technique to measure the thermo-physical properties of the cryo-protective agent was investigated.This study demonstrates that the biochip with integrated automatic loading and the inspection units can make it possible for massive optimization on the complex cryopreservation program to be a much quick and economic way.
【Fund】: 国家自然科学基金(50575219及50436030)资助项目
【CateGory Index】: TB611
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