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《Chinese Journal of Control of Endemic Disenaces》 2002-01
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Comparing of different methods for testing plague in rats tissues

ZHANG Hong-ying,WU Ming-shou,Guo Ying,et al (Yunnan Provincial Institute of Epidemic Disease Control and Research,Dali,Yunnan 671000,China)  
This paper reported that the double primers(pla-F1)PCR,bacteria growth and the indiect hemagglutination test (RIHA) were applied in 9 materials of rats of plague(6 guinea pigs,3 small white rats) for different preserving times.The results showed that all samples were positive detected by PCR and RIHA methods after preserving 90 and 180 days under 4℃.The positive rate of the bacteria nurture was lower.The PCR and RIHA methods more sensitivety than growth.Suggestion,when detected the materials of plague or suspect plague,can using PCR and RIHA methods besides with the growth to avoid the diagnostics mistake.
【Fund】: 云南省应用基础研究项目资助课题 (98C1 1 0M)
【CateGory Index】: R184
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