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《Chinese Journal of Control of Endemic Disease》 2003-01
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Experimental research on myocardial injury and mechanism of apoptosis induced by CVB_4' in Se-deficient mice

CAO Dan-yang,ZhONG Xue Ruan,LIU Yi,et al (General Hospital of Beijing Army,Beijing,China)  
Objective To investigate the mechanism of Se-deficient mice's myocardial injury and apoptosis induced by CVB4'.Methods The original Kunming mice were fed with Se-supplemented feed and Se-deficient feed respectively and mated after four weeks.The four-week male mice were inoculated intraperitoneally with CVB4',while control group were inoculated with equal-quantity RPMI 1 640 in the same way.Myocardial changes of mice in four groups were observed through light microscope,and the four-week mice's hearts were collected as experimental materials to examine apoptosis and it's related gene expression by using TUNEL and immunohistochemistry methods.Results The detection rate of myocardial lesion in Se-deficient virus group was 78.1%,and that of Se-supplemented virus group was 16.7%.The myocardium of the Se-deficient and Se-supplemented groups had no pathological changes.The myocardium of the Se-deficient virus group,Se supplemented virus group and Se-deficient groups had all apoptosis in different degree.There was no apoptosis in the rich selenium mice's myocardium.Conclusion Myocardial mechanism of apoptosis was involved in myocardial injury induced by CVB4' in Se-deficiency Kunming mice.