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《Chinese Journal of Control of Endemic Disease》 2003-01
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Observation of application effect of peripheral-blood PCR assay in diagnosis of brucellosis

WANG Ji-qiu, LI Tie-feng, ZHANG Gui-zhen ,et al (The Base for Control and Prevention of Plague and Brucellosis, the Chinese Center for Disease Control and Prevention , Baicheng 137000)  
Objective To follow up the potential role of PCR assay was used for diagnose brucellosis . Mothod We examined peripheral blood from acute and chronic brucellosis (50 cases) and health persons (45 cases) and the cavies after infected brucella,By PCR assay of contrivable three pair primers of the same protein gene sequence encoding immunogenetic 31-KD Br.abortus protein and its amplication fragment are 330bp,230bp and 223bp respectively, Result The results of our PCR assay were positive for 42 cases of acute and chronic brucellosis and five health persons; all of infected cavies were positive . Conclusion PCR assay on diagnosis brucellosis patients was upper sensitivity and certain specifity,its uses foreground is better.
【CateGory Index】: R516.7
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 Tang Liuying,et al The Institute of Epidemiology and Microbiology , Chinese Academy of Preventive Medicine;DETECTION OF BRUCELLA USING MOIECUIAR TECHNIQUES (I)DETECTION OF BRUCELLA BY PCR[J];Chinese Journal of Control of Endemic Disease;1995-04
2 Tang Liuying ,et al Department of Epidemiolog y ,Microbiology of Chinese Preventive Medical Sciences;DETECTION OF BRUCELLA USING MOLECULAR TECHNIQUES : II DIAGNOSIS OF ANIMAL BRUCELLOSIS BY PCR[J];Chinese Journal of Control of Endemic Disease;1997-04
3 LI Tie -feng,WANG Ji -qiu,GANG Sen-lin,et al(The First Institute of Endemic Disea se of Jilin Province,Baicheng 137000);A study of detecting Brucella DNA by PCR[J];Chinese Journal of Control of Endemic Disenaces;2002-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Ping1,YANG Guo-lin1,CHEN Da-jian2(1.Jiangsu Polytechnic College of Agricultural and Forestry,Zhenjiang,Jiangsu 212400 China;2.Jiangsu Runkang Pharmaceutical Co.,ltd.,Yanchen Jiangsu 45000,China);Advance in Brucellosis of Cows[J];china Cattle Science;2008-05
2 Tang Liuying ,et al Department of Epidemiolog y ,Microbiology of Chinese Preventive Medical Sciences;DETECTION OF BRUCELLA USING MOLECULAR TECHNIQUES : II DIAGNOSIS OF ANIMAL BRUCELLOSIS BY PCR[J];Chinese Journal of Control of Endemic Disease;1997-04
3 Shang Deqiu et al Institute of Epidemiology and Microbiology, Chinese Academy Preventive Medicine, Beijing;A STUDY ON THE NEW METHODS OF DIAGNOSIS FOR BRUCELLOSIS[J];Chinese Journal of Control of Endemic Disease;1999-03
4 Li Lanyu,Qiu HaiYin, Shang DaQiu (Institute of Epidemilogy and Microbiology, Chinese Academy Preventwe Medicine, Beijing, 102206);A study on the PCR about primers of 31KD_a protein gene for B.abortus[J];Chinese Journal of Control of Endemic Disenaces;2000-04
5 LI Lan-yu,QIU Hai-yan,SHANG De-qiu,et al (Institute of Epidemiology and Microbiology,Chinese Academy preventive Medicine,Beijing,102206);A study on the identification diagnosis of infection about Brucella SPP and Yersinia enterocolitia(Ⅱ)[J];Chinese Journal of Control of Endemic Disenaces;2002-01
6 LI Tie -feng,WANG Ji -qiu,GANG Sen-lin,et al(The First Institute of Endemic Disea se of Jilin Province,Baicheng 137000);A study of detecting Brucella DNA by PCR[J];Chinese Journal of Control of Endemic Disenaces;2002-04
7 WANG Li, MA Guo-zhu (Shaanxi Provincial Centers for Disease Control and Prevention, Xi'an ,710054);The research for diagnosing brucellosis patients with PCR technology[J];Chinese Journal of Control of Endemic Disease;2004-02
8 PIAO Dong-ri,ZHAO Hong-yan,LI Lan-yu,et al(National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206);A identification report on two strains of B. canis isolated from certain place of Beijing[J];Chinese Journal of Control of Endemic Diseases;2006-05
9 ZHAO Zhen-xiang,LI Lan-yu,PIAO Dong-ri,et al(Department of Microbiology and Immunology,Shanxi Medical University,Taiyuan 030001);PCR Assay for Detection of Brucella[J];Chinese Journal of Control of Endemic Diseases;2008-04
10 MA Ming-xiao~1 LIU Kai~2,YU Yang~1,ZHOU Tie-zhong~1,ZHANG Li-bo~1(1.Jinzhou Medical College,Jinzhou 121001 China;2.Inner Mongolia University for Nationalities,Tongliao 028000 China);Establishment of Rapid Test of Brucella by Nest-PCR[J];Journal of Jinzhou Medical College;2006-05
【Co-references】
Chinese Journal Full-text Database 6 Hits
1 Shang Deqiu et al Institute of Epidemiology and Microbiology, Chinese Academy Preventive Medicine, Beijing;A STUDY ON THE NEW METHODS OF DIAGNOSIS FOR BRUCELLOSIS[J];Chinese Journal of Control of Endemic Disease;1999-03
2 LI Tie -feng,WANG Ji -qiu,GANG Sen-lin,et al(The First Institute of Endemic Disea se of Jilin Province,Baicheng 137000);A study of detecting Brucella DNA by PCR[J];Chinese Journal of Control of Endemic Disenaces;2002-04
3 WANG Li, MA Guo-zhu (Shaanxi Provincial Centers for Disease Control and Prevention, Xi'an ,710054);The research for diagnosing brucellosis patients with PCR technology[J];Chinese Journal of Control of Endemic Disease;2004-02
4 DU Xin-ying,CHEN Ze-liang,WANG Yu-fei,QIAO Feng,ZHAO Jin,YUAN Xi-tong,HUANG Liu-yu(Institute of Disease Control and Prevention,Academy of Military Medical Science,Beijing 100071,Chian);DEVELOPMENT OF A MULTIPLEX PCR FOR BRUCELLA DETECTION[J];Journal of Preventive Medicine of Chinese People's Liberation Army;2008-05
5 Tang Liu-ying; Qiu Hai-yan; Li Yuan-kai; et al.(Institute of Epidemiology & Microbiblogy,Chinese Academy Preventive Medicine, Beijing 102206);The Epidemiologic Value of Polymerase Chain Reaction to the Diagnosis of Brucellosis Patients[J];CHINESE JOURNAL OF EPIDEMIOLOGY;1997-03
6 CHEN Wei-ye, WANG Shu-jie, HU Sen, WANG Yong, WANG Qing-hua, XIN Jiu-qing, TONG You-en, HUANG Ke-he, BU Zhi-gao(National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China);Recombinant BP26 and OMP31 proteins of Brucella used as the diagnostic antigen in indirect ELISA test[J];Chinese Journal of Zoonoses;2006-06
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Tang Liuying,et al The Institute of Epidemiology and Microbiology , Chinese Academy of Preventive Medicine;DETECTION OF BRUCELLA USING MOIECUIAR TECHNIQUES (I)DETECTION OF BRUCELLA BY PCR[J];Chinese Journal of Control of Endemic Disease;1995-04
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