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《Chinese Journal of Control of Endemic Disease》 2005-06
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A study of Dot Immunogold Filtration Assay-Thin Layer Chromatography for the detection of Y.pestis

YUE Ming-Xiang,LIU Nan,BAO Jian-Xi,et al(Institute of Hygiene and Environmental Medicine,Academic of Military Medical Science,Tianjin 300050)  
Objective A rapid,simple detective method-dot immunogold filtration assay-thin layer chromatography(DIGFA-TLC)for quantitative detecting of Plague was established in this experimental study.Methods 10nm colloidal gold marked polyclonal anti-PlagueF1 was prepared by reduction process with sodium citrate to fabricate a probe of anti-PlagueF1 colloidal gold for quantitative measurement of the immuno dot of sample with TLC.Results The detective limit of F1-antigen by DIGFA-TLC was as low as 5ng and that stains of EV76 was 1.2×105 cfu;furthermore,high positive correlation was between them.The correlative linear equations were y=448.467 37+2.511 43x,P0.01 and y=375.760 88+4.112 31×10~(-4)x,P0.05 respectively.The whole reactive process could be finished within 10 min.Conclusions The DIGFA-TLC is a simple, rapid,specific,sensitive and reliable assay,and quantitative detection of the Plague can be realized.
【Fund】: 国家自然科学基金项目(No.30371218)
【CateGory Index】: R446.6
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