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《Chinese Journal of Control of Endemic Diseases》 2009-01
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The method established for studying mechanism of Coptis Rhizome against Yersina pestis based on whole genome DNA microarray

ZHANG Jing-ling,BAI Qun-hua,JIA Yan,et al(School of Public Health,Chongqing Medicial University,Chongqing 400016)  
Objective To establish a method for studying molecular mechanism of Traditional Chinese Medicine Coptis rhizome against Y.pestis based on whole genome DNA microarray.Methods The MIC of Coptis rhizome to Y.pestis was determined by liquid dilution method.A whole genome DNA microarray that contains 4 005 annotated genes of Y.pestis was used.The gene expression profile of Y.pestis was performed after exposured to Coptis rhizome at the concentration of 10×MIC for 30 minutes.The total RNA extracted and purified from Y.pestis were reverse-transcribed to cDNA and labeled by Cy3-Cy5 dye.The labeled probes were hybridized to the microarray and the results were obtained by a laser scanner and analyzed by the SAM software.Results The platform of the DNA microarray-based bacteria transcriptional profiling was eshtablished.Conclusions The whole genome DNA microarray can be used in the studing of molecular mechanism of Coptis rhizome against Y.pestis.
【Fund】: 重庆医科大学科技启动基金资助
【CateGory Index】: R285.5
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 WU Ming-shou, ZHANG Hong-ying, DONG Xing-qi, WANG Peng, YIN Jia-xiang. Yunnan Institute for Endemic Disease Control and Prevention, Dali 671000, China;The PCR analyzing for plasmid pYC of Yersina pestis in China[J];Chinese Journal of Endemiology;2006-03
2 WU Zheng-jun (General Hopital of PLA, Beijing 100853, China);Anti-infective Treatment with Chinese Traditional Medicine[J];Chinese Journal of Nosoconmiology;2004-11
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Hong-ying, DONG Xing-qi, GUO Ying, WU Ming-shou (Yunnan Institute of Endemic Diseases Control and Prevention, Dali, Yunnan 671000,China);Application of Yersinia pestis Label Gene of Plasmid pYC in Detecting Animals and Fleas[J];Endemic Diseases Bulletin;2009-01
2 Huang Fajiang,Chen Jianqing,Pan Yimin,et al.Pujiang People's Hospital,Pujiang 322200,Zhejiang,China;Change in plasma tumor necrosis factor-α and interleukin-6 levels after intraperitoneal infection under treatment of combining antibiotics with traditional Chinese medicine in rat[J];Infection,Inflammation,Repair;2007-02
3 YAN Xiao-juan,ZHANG Jing-ling,YANG Jing-fang,et al.(College of Public Health,Chongqing Medical University,Chongqing 400016,P.R.China);Antibacteral effect of six traditional Chinese medicines against methicillin-resistant Staphylococcus aureu[J];China Tropical Medicine;2009-02
4 ZENG Zhi-hong1,HE Jian-ren2(1.Environment & Life Sciences Department,Putian University,Putian Fujian 351100,China;2.Putian Entry-exit Inspection and Quarantine Bureau,Putian Fujian 351100,China);Primary Study on Antibacteral Effect of Three Common Plants[J];Journal of Putian University;2009-05
5 WU Zheng-jun,CHEN Li-ping(General Hospital of PLA,Beijing 100853,China);Treatment of Carcinoma Fever by Traditional Chinese Medicine[J];Chinese Journal of Nosoconmiology;2005-10
6 HAO Ai-zhen,CHEN Li-ping,GAO Li-ping(General Hospital of PLA,Beijing 100853,China);Recurrent Respiratory Tract Infections Treatment by Traditional Chinese Medicine[J];Chinese Journal of Nosocomiology;2006-01
7 CHEN Li-ping,WU Zheng-jun(General Hospital of PLA,Beijing 100853,China);Influence of Monograph Treatise on Pestilence Dealing with Infectious Disease on Traditional Chinese Medicine[J];Chinese Journal of Nosocomiology;2006-05
8 WU Zheng-jun, CHEN Li-ping, ZHANG Jun-ming (General Hospital of PLA,Beijing 100853, China);Antibiotic Effect of Formula of Qinwenbaiduyin:An Experimental Study[J];Chinese Journal of Nosocomiology;2006-09
9 XING Jin-yan ZHANG Shun SUN Yun-bo Department of ICU,the Affiliated Hospital of Medical College of Qingdao University,Oingdao 266003,China;Effect of traditional Chinese medicine on the cellular immunity of severe acute pancreatitis patients[J];Chinese Journal of Critical Care Medicine;2007-04
10 XUE Jian-jiang1,QIAO Hai-xia2,LIU Jin-jun2,et al 1.Clinicial Lab,The First Affilited Hospitial,Hebei North University,Zhangjiakou,075000,Hebei,China 2.Department of Microbiology,Hebei North University,Zhangjiakou,075000,Hebei,China;Study on Antibacteral Effects of Six Chinese Herbs Against Pathogenic Bacteria[J];Journal of Hebei North University(Medical Edition);2008-03
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 Dongxing-qi,et al(Yunnan provincial institute for Epidemic Disease control);Discovery and identification of 4 Mdal plasmid of Y.pestis[J];Chinese Journal of Endemiology;1993-04
2 WANG Zhu-yun * ,LUO Song Da-wei,YU Xiao-tao,CHEN Hong-jian,YUE Shan-long,WANG Yuan-zhong,LI Min,QI Zhi-zhen,CUI Bai-zhong,LI Chao,LI Min,YU Shou-hong,XIN You-quan,DA De La-jia,DING Xian-hua.* Qinghai Institute For Endemic Disease Prevention and Control,Xining811602,China;The discovery and research of Microtus fuscus plague natural foci in Qinghai province[J];Chinese Journal of Endemiology;2004-01
3 DONG Xingqi, YE Feng, PENG Hebi, et al.Yunnan Institute of Epidemic Diseases Control and Research, Dali 671000,China;Geographic distribution and feature of Yersinia pestis plasmid isolated from Yunnan province[J];Chinese Journal of Epidemiology;2001-05
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