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《Chinese Journal of Control of Endemic Diseases》 2009-01
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The method established for studying mechanism of Coptis Rhizome against Yersina pestis based on whole genome DNA microarray

ZHANG Jing-ling,BAI Qun-hua,JIA Yan,et al(School of Public Health,Chongqing Medicial University,Chongqing 400016)  
Objective To establish a method for studying molecular mechanism of Traditional Chinese Medicine Coptis rhizome against Y.pestis based on whole genome DNA microarray.Methods The MIC of Coptis rhizome to Y.pestis was determined by liquid dilution method.A whole genome DNA microarray that contains 4 005 annotated genes of Y.pestis was used.The gene expression profile of Y.pestis was performed after exposured to Coptis rhizome at the concentration of 10×MIC for 30 minutes.The total RNA extracted and purified from Y.pestis were reverse-transcribed to cDNA and labeled by Cy3-Cy5 dye.The labeled probes were hybridized to the microarray and the results were obtained by a laser scanner and analyzed by the SAM software.Results The platform of the DNA microarray-based bacteria transcriptional profiling was eshtablished.Conclusions The whole genome DNA microarray can be used in the studing of molecular mechanism of Coptis rhizome against Y.pestis.
【Fund】: 重庆医科大学科技启动基金资助
【CateGory Index】: R285.5
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