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《Chinese Journal of Control of Endemic Diseases》 2012-01
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Effects of inorganic arsenic on the protein expressions of Nrf2 and its regulated downstream antioxidase

LI Bing,XING Xiao-yue,LI Wei,LIU Dan,WANG Xin,LI Xin,SUN Gui-fan Department of Occupational and Environmental Health,Liaoning Provincial Key Laboratory of Arsenic Biological Effect and Poisoning,School of Public Health,China Medical University,Shenyang,Liaoning 110001,China  
Objective To observe the effects of inorganic arsenic on the protein expressions of transcription factor Nrf2 and its regulated downstream antioxidase in keratinocyte.Methods Human keratinocyte HaCaT cell line was exposed to sodium arsenite(NaAsO2,25 μmol/L) for 0.5 h,3 h,6 h,12 h and 24 h,and western blot assays were used to detect the protein expression of Nrf2,NQO1 and HO-1.Results 25 μmol/L of NaAsO2 exposure could significantly induced the protein expressions of Nrf2 at 0.5 h,3 h,6 h,12 h and 24 h,respectively(P0.01).Nrf2 protein levels kept higher at 3 h,6 h and 12 h,while decreased at 24 h,but still higher notably than control group.NQO1 protein levels went higher at only 3 h after exposure(P0.01),while significantly decreased at 6 h,12 h and 24 h(P0.05 or 0.01).By contrast,HO-1 protein expressions were induced at 3 h obviously,and kept on increasing at 6 h,12 h and 24 h,with a clear time-effect response.Conclusion The protein expressions of Nrf2 and its regulated downstream antioxidase NQO1 and HO-1 could be induced by inorganic arsenic in human keratinocyte HaCaT cells.
【Fund】: 辽宁省教育厅科学技术研究项目(L2010704)
【CateGory Index】: R114
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 LI Bing, LIU Jie, LI Xin, et al. Department of Occupational Health, School of Public Health, China Medical University, Shenyang, Liaoning 110001, China;Effects of Sodium Arsenite on Expression of Transcription Factor Nrf2 and Heme Oxygenase 1 in Chang Liver Cells[J];Journal of Environment and Health;2009-12
【Co-citations】
Chinese Journal Full-text Database 1 Hits
1 LI Bing,LI Xin,ZHU Bo,ZHANG Xin-yu,LIU Dan,XING Xiao-yue,WANG Xin,ZHANG Qi, SUN Gui-fan(Department of Occupational Health,Key Laboratory of Arsenic-related Biological Effects, Prevention and Treatment in Liaoning Province,School of Public Health,China Medical University, Shenyang,Liaoning 110001,China);Effects of Inorganic Arsenic on mRNA and Protein Expression of Heme Oxygenase-1 in Chang Hepatocytes[J];Journal of Environmental & Occupational Medicine;2011-08
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