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《Chinese Journal of Control of Endemic Diseases》 2012-03
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An analysis on fluorine-induced autophagy and apoptosis of MC3T3-E1 and their interaction

GUO Xiao-dong,YANG Mao-wei,LIANG Dan,GUO Bao-lei,YANG Lei,CAO Jun-jun The First Hospital of China Medical University,Shenyang 110001,China  
Objectives: To explore Fluorine-induced apoptosis and autophagy of MC3T3-E1 cells and the relationship between them.Methods To explore the concentration of fluorine that caused cell apoptosis the influence of fluorine on proliferative vitality of MC3T3-E1 was assessed by MTT assay.The apoptosis of MC3T3-E1 was detected by flow cytometry;The expression of apoptosic protein was determined by Western Blot;The LC3 and the Beclin1 protein level and variation were analyzed by western blot assay.Results Fluorine inhibited the proliferation of MC3T3-E1 cells dose and time dependently and simultaneously induced apoptosis and autophagy;Inhibition of autophagy by 3-MA promotes fluorine-induced apoptosis.Conclusion Fluorine can inhibit cell proliferation and induce apoptosis and autophagy,but autophagy partly atagonizes apoptosis.
【CateGory Index】: R151
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Chinese Journal Full-text Database 1 Hits
1 GUO Xiao-ying,CAI Ruo-xin,HE Yang, et al. School of Public Health,China Medical University,Shenyang,Liaoning 110001,China;Effect of Fluoride on Mineralization in Mice Osteoblast Cell Line MC3T3-E1 Cells[J];Journal of Environment and Health;2010-08
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Chinese Journal Full-text Database 3 Hits
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