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《Chinese Journal of Control of Endemic Diseases》 2012-03
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Pulse-field gel electrophoresis test of Yersini pestis vaccine strain

SHI Li-yuan,WANG Peng,YE Rui,ZHANG Rong Institute of Endemic Diseases Control and Prevention of Yunnan Province,Dali 671000,China  
Objective To form standard operation procedure of pulse-field gel electrophoresis of Yersini pestis and to make risk evaluation.Methods Vaccine strain EV76 was applied in PFGE.Electrophoresis patterns of two endonucleases were comgared,observed influence of different culture time and detected germs in plugs.Results Culture time had no influence on the pattern,FseI bands were easier to be recognized and there was no germ in plugs.Conclusion To ensure laboratory biosafety in the operation of PFGE standard operation procedure must be strictly carried out.
【Fund】: 国家自然科学基金项目(81160354)
【CateGory Index】: R392
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【Citations】
Chinese Journal Full-text Database 2 Hits
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【Co-citations】
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【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 WEI Jian chun, HAI Rong, SUN Lian zhi (1.Institute for Infective Disease, Chinese Center for Disease Control and Prevention, Beijing 102206, China; 2.Inner Mongolia Institute for Epidemic Disease Control, Huhehaote 010031, China);Pulse-field gel electrophoresis of Yersinia pestis that isolated from Shiqu county, Sichuan province, China.[J];Chinese Jouranl of Endemiology;2002-04
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