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《Chinese Journal of Control of Endemic Diseases》 2012-03
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Pulse-field gel electrophoresis test of Yersini pestis vaccine strain

SHI Li-yuan,WANG Peng,YE Rui,ZHANG Rong Institute of Endemic Diseases Control and Prevention of Yunnan Province,Dali 671000,China  
Objective To form standard operation procedure of pulse-field gel electrophoresis of Yersini pestis and to make risk evaluation.Methods Vaccine strain EV76 was applied in PFGE.Electrophoresis patterns of two endonucleases were comgared,observed influence of different culture time and detected germs in plugs.Results Culture time had no influence on the pattern,FseI bands were easier to be recognized and there was no germ in plugs.Conclusion To ensure laboratory biosafety in the operation of PFGE standard operation procedure must be strictly carried out.
【Fund】: 国家自然科学基金项目(81160354)
【CateGory Index】: R392
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 XIA Lian-xu, HAI Rong, WEI Jian-chun, et al (The Research Center of Endemic Disease Control of Nei Monggol Region,Huhhot 010020);Analysis of the molecular karotypes in Yersinia pestis by Pulse-field gel electrophoresis[J];Chinese Journal of Control of Endemic Disease;2003-06
2 WEI Jian chun, HAI Rong, SUN Lian zhi (1.Institute for Infective Disease, Chinese Center for Disease Control and Prevention, Beijing 102206, China; 2.Inner Mongolia Institute for Epidemic Disease Control, Huhehaote 010031, China);Pulse-field gel electrophoresis of Yersinia pestis that isolated from Shiqu county, Sichuan province, China.[J];Chinese Jouranl of Endemiology;2002-04
【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 XIA Lian-xu, HAI Rong, WEI Jian-chun, et al (The Research Center of Endemic Disease Control of Nei Monggol Region,Huhhot 010020);Analysis of the molecular karotypes in Yersinia pestis by Pulse-field gel electrophoresis[J];Chinese Journal of Control of Endemic Disease;2003-06
2 ZHOU Shu-wu, YANG Jin-ye. (Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention, Nanning 530021, Guangxi, P. R. China);Advance in the research of genetic typing of Yersinia pestis[J];China Tropical Medicine;2007-03
3 WANG Peng,SHI Li-yuang,DONG Xing-qi,YU Guo-lin.Yunnan Institute for Endemic Diseases Control and Prevention Dali,Yunnan 671000);Pulsed Field Gel Electrophoresis fingerprinting of Yersinia pestis in Yunnan[J];Chinese Journal of Pest Control;2006-05
4 CHEN Gui-chun*, HAI Rong, ZHANG Zhi-kai, DONG Li, YAO Guan-hai, GONG Xiao-jun, LIU Zhao-bing, HUANG Hong-wu. *Guizhou Center for Disease Control and prevention, Guiyang 550004, China;Study on biological characteristics of Yersinia pestis in Guizhou[J];Chinese Journal of Endemiology;2005-02
5 HAI Rong;YU Dong-zheng;WEI Jian-chun;XIA Lian-xu;SHI Xian-ming;ZHANG Zhi-kai;ZHANG En-min Institute for Infectious Disease Control and Prevention, Chinese Center for Disease Control and Prevention,Beijing 102206, China;Molecular biological characteristics and genetic significance of Yersinia pestis in China[J];Chinese Journal of Epidemiology;2004-06
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 Ji Shuli,et al;A Study on Typing of Y.Pestis in China and Ecologico-epidemiological Significance[J];Chinese Journal of Endemiology;1987-05
2 WEI Jian chun, HAI Rong, SUN Lian zhi (1.Institute for Infective Disease, Chinese Center for Disease Control and Prevention, Beijing 102206, China; 2.Inner Mongolia Institute for Epidemic Disease Control, Huhehaote 010031, China);Pulse-field gel electrophoresis of Yersinia pestis that isolated from Shiqu county, Sichuan province, China.[J];Chinese Jouranl of Endemiology;2002-04
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