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《Chinese Journal of Control of Endemic Diseases》 2012-03
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An in vivo study on the effect of ILK antisense oligonucleotide on anti-xenograft of human epithelial ovarian cancer

LI Qi,LI Chen,CHEN Wei,LV Jun-li,SUN Jing Harbin Medical University Cancer Hospital,Harbin 150081,China  
Objective To explore the inhibiting of transfection of integrin-linked kinase antisense oligonucleotides(ILK-ASODN) on the development of subcutaneous xenograft of human epithelial ovarian cancer in nude mice,and therapeutic effect on treatment of the developed subcutaneous xenograft.Methods This study included two parts.The first part was subcutaneous inoculation of human ovarian cancer cell lines(HO-8910) with transfection of ILK-ASODN to the nude mice in the experimental group,and subcutaneous inoculation of HO-8910 without transfection of ILK-ASODN to the nude mice in the control group to observe the changes of weight of nude mice,time for tumor development,changes of tumor volume and weight,and calculated the inhibitory rates.The second part was subcutaneous inoculation of the HO-8910 cells in nude mice to establish the model of human ovarian cancer in nude mice.Then the mice were randomly divided into treatment group and control group.After each intraperitoneal injection of the treatment,the weights of the mice,the weight and volume of the tumors in nude mice were recorded,and the rates of tumor inhibition were calculated.Results In comparison with the control group,tumors of the nude mice in the experimental group of the first part of the study developed statistically significant later(P0.01) and grew statistically significant slower in volume(P0.01) and in weight(P0.01).In comparison with the control group,tumors of the nude mice in the treatment group of the second part of the study grew in volume and weight much slower,showing statistically significant(P0.01).Conclusion Our study showed ILK-ASODN significantly inhibited the development,and the growth of the developed xenografts of human ovarian cancer in nude mice,indicating a preventive and therapeutic effect.
【Fund】: 黑龙江省青年科学基金项目(QC2010067)
【CateGory Index】: R737.31
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