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《Chinese Journal of Control of Endemic Diseases》 2012-06
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Antagonism of tBHQ on HaCaT cells apoptosis induced by NaAsO_2

LI Wei,LIU GUO-Jie,XING Xiao-yue,DONG Dan-dan,NIE Hui-fang,LI Bing Department of Occupational and Environmental Health,Liaoning Provincial Key Laboratory of Arsenic Biological Effect and Poisoning,School of Public Health,China Medical University,Shenyang,Liaoning 110001,China  
Objective To discuss the antagonistic effect of tertiary butylhydroquinone(tBHQ) on human keratinocytes cells(HaCaT) apoptosis induced by sodium arsenite(NaAsO2).Methods HaCaT cells was pretreated with tBHQ(10,25 and 50 μmol/L) for 12 hours,then was treated together with NaAsO2(5,10 and 30 μmol/L) for 24 hours.The rate of cell apoptosis was determined by Annexin V/PI staining flow cytometry;the morphology was evaluated by fluorescence microscopy following DAPI staining.Mitochondrial membrane potential was determined by JC-1 method.Result The results of ANOVA test indicated the rate of apoptosis significantly increased treated with NaAsO2(5,10 and 30 μmol/L) compared with control group and the depolarization of mitochondrial membrane potential gradually decreased compared with the control group(P0.05 or P0.01).The morphology showed the number of staining cells was significantly increased and apoptotic body appeared in high strength DAPI staining.The increased rate of HaCat cell apoptosis and decreased mitochondrial membrane potential were inhibited in the condition of pretreating with tBHQ(10,25 and 50 μmol/L)(P0.05).The morphology showed the number of staining cells and cell debris were significantly reduced.Conclusions The results of the study indicate that tBHQ inhibits HaCaT cells apoptosis induced by NaAsO2 by mitochondrial apoptotic pathway.
【Fund】: 国家自然科学基金项目(编号:81172611);; 辽宁省教育厅资助项目(L2010704)
【CateGory Index】: R114
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