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《Chinese Journal of Control of Endemic Diseases》 2014-01
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Influence of low level and long-term arsenic exposure to the phosphorylated protein kinase B as well as its downstream signal factor IKK,I-ΚB and NF-κB in HaCat cells

LIU Shi-yi;YIN Xiu-yan;CAI Si-si;HU Nan-nan;YI Qiu-rong;LI Xin;Department of ℃ cupational Health,School of Public Health,China Medical University;  
Objective To discuss the influence of low level and long-term arsenic exposure to the phosphorylated protein kinase B as well as its downstream signaling factor IKK,I-ΚB and NF-κB in HaCat cells. Methods HaCat cells were exposed to NaAsO 2 with different concentration of 0,0. 05,0. 1 μmol / L for 15 weeks,while the protein expression levels of p-Akt,phosphorylated IKK( p-IKK),phosphorylated IκB( p-IκB) and NF-κB were detected by Western Blot. Results Protein levels of pAkt,p-IKK and p-IκB of the 0. 05 and 0. 1 μmol / L groups were significantly higher than that of the control group; protein level of NF-κB in nuclear of the 0. 05 and 0. 1 μmol / L groups were significantly higher than that of control,while its level in cytoplasm were markedly lower than that of control. Conclusion Low level and long term arsenic exposure induces protein phosphorylation of p-Akt in HaCat cells,which result in phosphorylated activation of its downstream signal factor IKK,IκB and expression of NF-κB in the nuclear.
【Fund】: 辽宁省教育厅课题(L2010705)
【CateGory Index】: R730.2
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【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 Yang Bei;Chen Xue;Ding Jinlan;Department of Histology and Embryology,College of Basic Medical Sciences,China Medical University;;Effect of RNAi-mediated Nrf2 knockdown on sodium arsenite-induced cytotoxicity in mouse pancreatic β cells[J];Chinese Journal of Histochemistry and Cytochemistry;2013-05
2 SONG Ying-li;XI Shu-hua;School of Public Health,China Medical University;;Mechanisms of cell malignant transformation during chronic arsenic expoure-induced cell transformation: a review of recent studies[J];Journal of Environment and Health;2013-10
3 LI Xin,LIU Shi-yi,CUI Yan,LI Bing,SUN Gui-fan;Effect of sodium arsenite on cell viability and phosphorylated protein kinase B expression in HaCat cells[J];Chinese Journal of Industrial Medicine;2011-06
4 LI Ying-na;ZHANG Lin;LI Xiu-li;ZHENG Hua-dong;YU Jun-xia;QIN Xu-jun;YANG Wei-lin;Department of Geriatrics, the Second Affiliated Hospital, Xi'an Jiaotong University;Department of Toxicology, the Key Laboratory of Free Radical Biology and Medicine of Shaanxi Province, the Ministry of Education Key Laboratory of Hazard Assessment and Control in Special Operational Environment, School of Preventive Medicine, the Fourth Military Medical University;;The Role of NADPH Oxidase in the Oxidative Stress of Human Liver Cells Induced by Arsenic Exposure[J];Progress in Modern Biomedicine;2013-27
5 LIU Shi-yi,LI Xin(Department of Occupational Health,School of Public Health,China Medical University,Shenyang,Liaoning Province 110001,China);Influence of low level,long-term arsenite exposure on cell cycle,cyclin D1 and p21 of HaCat cells[J];Chinese Journal of Public Health;2013-08
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1 Xuejun Jiang,Zunzhen Zhang~* (Department of Environmental Health,West China School of Public Health, Sichuan University,Chengdu,610041,PR.China.);Sodium arsenite and arsenic trioxide differently affect the oxidative stress,genotoxicity and apoptosis in A549 cells:an implication for the paradoxical mechanism[A];[C];2013
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