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《Chinese Journal of Control of Endemic Diseases》 2017-08
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Mechanism of induced pluripotent stem cell-derived cardiomyocytes on cardiac function after myocardial infarction in rats

JIANG Xin;LUO Te-dan;LI Peng;The Second Clinical Medical College of Jinan University,Shenzhen People's Hospital;  
Objective The purpose of this study is to investigate the mechanism of heart function improvement using human induced pluripotent stem cells( i PSC-CMs) for myocardial repair in a rat model of myocardial infarction( MI).Methods Animals induced with MI model by LAD ligation were randomly assigned to receive direct intramyocardial injection of 4 × 105 i PS-CMs( i PS-CMs group) or PBS( MI group) at the infarct and border zone. The heart function changes,myocardial cell apoptosis and proangiogenic proteins expression was evaluated 4 weeks after i PS-CMs transplantation.Results The LVEF was significantly increased and native cardiomyocytes apoptosis was significantly reduced in i PS-CMs group compared with the MI group( 52.7 ± 4.52% vs 41.82 ± 6.31%,and 29.33±9.86% vs 44.63± 10.25%,n = 8,P 0. 05) 4 weeks after cell transplantation. ELISA result showed i PS-CMs transplantation can upgrade the expression of angiopoietin-1 protein and vascular endothelial growth factor( VEGF).Conclusion Our results suggest that transplantation of i PSC-CMs can improve the heart function via restrain the native cardiomyocytes apoptosis and upgrade the expression of the proangiogenic proteins after transplantation in MI rat models.
【Fund】: 国家自然科学基金资助项目(No.81401570);; 深圳市科技创新委资助项目(No.JCYJ 20130402101926972)
【CateGory Index】: R542.22
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