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《Progress in Veterinary Medicine》 1997-01
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Generically—specific Common Antigenic Epitopes on Salmonella flagellins and Their Immumobiological Properties

Jiao Xinan Liu Xiufin Wang Zhiliang Zhang Rukuan Wen Qiyi Cai Xuezhong Yang Jing Cao Junping Gao Song(Dept. of Vet. Sci. ,Yangzhou University,Yangzhou 225009)  
The multiple epitopes of generically-specific common antigen on Salmonella flagellins were defined by a panel of monoclonal antibodies in immunogold, Indirect immunofluorescent test, Western blotting and ELISAs. This result was further verified by detecting these epitopes on the expression product of flagellin gene fliCi in E. Coli Le—2a. The distribution of these epitopes was assessed by examining the binding patterns of each MAb to a set of 219 Salmonella strains and 892 isolates covering A through O—67 serogroups and 96 other enteric bacteria. The results showed that these common epitopes on Salmonella flagellin molecules could be used as a new recognized marker for the genus of Salmonella. These epitopes could be used for the identification of Salmonella isolates and rapid detection of Salmonella contamination in different specimens.
【CateGory Index】: S852.5
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