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《Progress In Veterinary Medicine》 2001-04
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Influence Factors on MEF Feeder Layer

YANG Qi 1,SHI Yuan gang 2,DOU Zhong ying 1 (1.Key laboratory of Animal Reproductive Endocrinology and Embryo Biotechnology of Agricultural Ministry of China,Northwest Sci Tech University of Agriculture & Forestry,Yangling,Shaanxi,712100 China; 2  
The influences of treating time with Mekemycin C,digesting time and concentration of feeding cells on MEF feeder layer were studied with murine embryos.The result showed as follows:①Treating with 10 μg/mL Mekemycin C,the time should be controlled within 1.5~2 h;②Digesting with 0.2% trypsin+0.04%EDTA,the time should be controlled within 2~3 min(37 ℃);③The concentration of feeding cells shhould be controlled withhin 3~3.5×10 5 mL -1 ;④During isolating MEF,the cells could be purified by changing Medium after 30 min feeding MEF on the plate.
【Fund】: 国家自然科学基金资助 (396 70 35 9);; 国家攻关课题 (96 - C0 1- 0 3)资助
【CateGory Index】: S852.1
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